| 英文名稱 | Neural Lineage Markers Antibody Assembly kit |
| 中文名稱 | 神經(jīng)譜系標(biāo)記抗體套裝 |
| 別 名 | Neural Lineage Markers Antibody Assembly kit(TUBB3,GFAP,MAP2,Nestin,NeuN,Vimentin) |
| 研究領(lǐng)域 | 神經(jīng)生物學(xué) 細(xì)胞類型標(biāo)志物 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Mouse, Rat, ) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | : |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | In central nervous system, neural stem cells (NSC) differentiate into multiple neural lineages, including neural precursors, astrocytes, neurons, and oligodendrocytes. Neuronal lineage markers are endogenous tags that are expressed in different cells along neurogenesis and differentiated cells such as neurons. We can use it to detect and identify different types of cells by different techniques. Neural Lineage Markers Antibody Assembly kit is one economical package of selected antibodies for neural lineage markers, including neural stem cell and progenitor marker Nestin, mature neuron marker NeuN and MAP2, Immature neuron maker TUBB3, radial glia cell marker Vimentin, mature astrocytes and other glial cell marker GFAP. These antibodies have been tested in a variety of applications and species. For specific application information of different antibodies, please refer to each individual antibody datasheet. Neural Lineage Markers Antibody Assembly kit Rabbit Anti-TUBB3 (Neuronal Marker) antibody (bs-4512R) Rabbit Anti-GFAP antibody (bs-0199R) Rabbit Anti-MAP2 antibody (bs-1369R) Rabbit Anti-Nestin antibody (bs-0008R) Rabbit Anti-NeuN antibody (bs-1613R) Rabbit Anti-Vimentin antibody (bs-0756R) Database links: Neural Lineage Markers Antibody Assembly kit Rabbit Anti-TUBB3 (Neuronal Marker) antibody (bs-4512R) Rabbit Anti-GFAP antibody (bs-0199R) Rabbit Anti-MAP2 antibody (bs-1369R) Rabbit Anti-Nestin antibody (bs-0008R) Rabbit Anti-NeuN antibody (bs-1613R) Rabbit Anti-Vimentin antibody (bs-0756R)
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 在中樞神經(jīng)系統(tǒng)中，神經(jīng)干細(xì)胞(NSC)可分化為多種神經(jīng)細(xì)胞類型，包括神經(jīng)前體、星形膠質(zhì)細(xì)胞、神經(jīng)元和少突膠質(zhì)細(xì)胞等。神經(jīng)譜系標(biāo)記是一些在神經(jīng)發(fā)生或分化后不同細(xì)胞類型中特征性表達(dá)的一些蛋白。我們利用可以這些特征性標(biāo)記通過(guò)不同的技術(shù)來(lái)檢測(cè)和識(shí)別不同類型的細(xì)胞。神經(jīng)譜系標(biāo)記抗體裝配試劑盒極具性價(jià)比，包含了最具代表性的神經(jīng)譜系標(biāo)記抗體，包括神經(jīng)干細(xì)胞和祖細(xì)胞標(biāo)記Nestin、成熟神經(jīng)元標(biāo)記NeuN和MAP2、未成熟神經(jīng)元標(biāo)記TUBB3、放射狀膠質(zhì)細(xì)胞標(biāo)記Vimentin、成熟星形膠質(zhì)細(xì)胞和其他膠質(zhì)細(xì)胞標(biāo)記GFAP。這些抗體在多種應(yīng)用和物種中進(jìn)行了測(cè)試。有關(guān)抗體具體使用信息，請(qǐng)參閱各個(gè)抗體產(chǎn)品數(shù)據(jù)表。 |
| 產(chǎn)品圖片 |  Primary: Anti-MAP2 (bs-1369R)Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP2) Polyclonal Antibody, Unconjugated (bs-1369R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Primary: Anti- GFAP (bs-0199R) Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GFAP Polyclonal Antibody, Unconjugated(bs-0199R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Primary: Anti-TUBB3 (Neuronal Marker) (bs-4512R) Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TUBB3/beta III Tubulin(Neuronal Marker) Polyclonal Antibody, Unconjugated(bs-4512R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Primary:Anti-NeuN(bs-1613R)Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (bs-1613R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.  Primary: Anti- Vimentin (bs-0756R)Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-0756R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.  Primary: Anti-Nestin Polyclonal Antibody, Unconjugated(bs-0008R) Primary:Anti-NeuN(bs-1613R)Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (bs-1613R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.  Primary: Anti-TUBB3 (Neuronal Marker) (bs-4512R)Tissue/cell: BV-2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (TUBB3) Polyclonal Antibody, Unconjugated (bs-4512R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.  Primary: Anti- Vimentin (bs-0756R)Tissue/cell: HeLa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-0756R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.  Primary: Anti-MAP2 (bs-1369R)Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP2) Polyclonal Antibody, Unconjugated (bs-1369R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.  Primary: Anti- GFAP (bs-0199R)Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4°C, followed by a conjugated secondary (bs-0295G-Cy3) at [1:500] for 90 minutes and DAPI staining of the nuclei.  Primary: Anti-Nestin Polyclonal Antibody, Unconjugated(bs-0008R)Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Nestin Polyclonal Antibody, Unconjugated(bs-0008R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei |
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