| 中文名稱 | 脂聯(lián)素受體2抗體 |
| 別 名 | Adipo R2; Progestin and adipoQ receptor family member II; Adipor2; PAQR2; ADR2_HUMAN. |
| 研究領域 | 細胞生物 免疫學 生長因子和激素 細胞膜受體 糖尿病 內分泌病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Dog, Pig, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 44kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Adiponectin receptor 2:315-340/386 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | The adiponectin receptors, ADIPOR1 (MIM 607945) and ADIPOR2, serve as receptors for globular and full-length adiponectin (MIM 605441) and mediate increased AMPK (see MIM 602739) and PPAR-alpha (PPARA; MIM 170998) ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin (Yamauchi et al., 2003 [PubMed 12802337]).[supplied by OMIM, Mar 2008] Function: Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some intermediate-affinity receptor activity for both globular and full-length adiponectin. Subunit: May form homo and heteromultimers. Subcellular Location: Membrane; Multi-pass membrane protein. Note=Localized to the cell membrane and intracellular organelles. Tissue Specificity: Highly expressed in skeletal muscle, liver and placenta. Weakly expressed in brain, heart, colon, spleen, kidney, thymus, small intestine, peripheral blood leukocytes and lung. Similarity: Belongs to the ADIPOR family. SWISS: Q86V24 Gene ID: 79602 Database links: Entrez Gene: 79602 Human Entrez Gene: 68465 Mouse Entrez Gene: 404694 Pig Entrez Gene: 312670 Rat Omim: 607946 Human SwissProt: Q86V24 Human
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 脂聯(lián)素與受體結合后具有增強胰島素敏感性，抗高血糖，抗動脈粥樣硬化等生物學效應，任何增加或減少脂聯(lián)素及其受體表達的方法都影響這些疾病的發(fā)生和發(fā)展。 脂聯(lián)素受體(AdipoR1和AdipoR2)同屬于PAQR家族，具有七次跨膜蛋白的特有結構. |
| 產品圖片 |  Sample: Muscle (Mouse) Lysate at 40 ugPrimary: Anti- Adiponectin Receptor 2 (bs-0611R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 44 kD  Sample:Lane 1: Heart (Mouse) Lysate at 40 ug Lane 2: Skeletal muscle (Mouse) Lysate at 40 ug Lane 3: Heart (Rat) Lysate at 40 ug Lane 4: Skeletal muscle (Rat) Lysate at 40 ug Lane 5: 293T (Human) Cell Lysate at 30 ug Primary: Anti-Adiponectin receptor 2 (bs-0611R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 43 kD  Sample:293T(Human) Cell Lysate at 30 ug Primary: Anti-Adiponectin receptor 2 (bs-0611R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 48 kD  Sample:muscle (Mouse) Lysate at 40 ug Primary: Anti-Adiponectin receptor 2 (bs-0611R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 44 kD  Sample:Lung (Mouse) Lysate at 40 ug Primary: Anti-Adiponectin receptor 2 (bs-0611R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 46 kD  Tissue/cell: human rectal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated(bs-0611R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated(bs-0611R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control: HepG2(blue).Primary Antibody:Rabbit Anti-Adiponectin receptor 2 antibody (bs-0611R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (bs-0611R, 0.2μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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