| 中文名稱 | 纖維狀肌動(dòng)蛋白抗體 |
| 別 名 | f actin; Filamentous actin; F-actin capping protein alpha subunit; CapZ alpha-1; CAZA1_HUMAN; CAPZA1. |
| 研究領(lǐng)域 | 免疫學(xué) 細(xì)胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, (predicted: Pig, Cow, Sheep, ) |
| 產(chǎn)品應(yīng)用 | WB=1:1000-5000 ELISA=1:500-1000 IHC-P=1:100-500 Flow-Cyt=3μg /test （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 42kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human F-actin capping protein alpha subunit:1-50/374 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | In vertebrates, three main groups of actin isoforms, alpha, beta, and gamma have been identified. The alpha actins, found in muscletissues, are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Individual subunits of microfilaments are known as globular actin (G-actin). G-actin subunits assemble into long filamentous polymers called F-actin. SWISS: P60709 Gene ID: 60 Database links: Entrez Gene: 60 Human Entrez Gene: 11461 Mouse Entrez Gene: 100009272 Rabbit Entrez Gene: 81822 Rat Omim: 102630 Human SwissProt: P60709 Human SwissProt: P60710 Mouse SwissProt: P29751 Rabbit SwissProt: P60711 Rat Unigene: 520640 Human Unigene: 708120 Human Unigene: 727576 Human Unigene: 328431 Mouse Unigene: 391967 Mouse Unigene: 94978 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 纖維狀肌動(dòng)蛋白F-Actin主要存在于橫紋肌原肌纖維細(xì)絲及平滑肌中，是肌肉運(yùn)動(dòng)和細(xì)胞運(yùn)動(dòng)的重要蛋白。 |
| 產(chǎn)品圖片 |  Sample: Lane1:Hela (Human) Whole Cell Lysate at 30 ugLane2:Jurkat (Human) Whole Cell Lysate at 30 ug Lane3:293T (Human) Whole Cell Lysate at 30 ug Lane4:Hepg2 (Human) Whole Cell Lysate at 30 ug Lane5:A431 (Human) Whole Cell Lysate at 30 ug Lane6:A549 (Human) Whole Cell Lysate at 30 ug Lane7:H9C2 (Rat) Whole Cell Lysate at 30 ug Lane8:Testis (Mouse) Tissue Lysate at 30 ug Primary: Anti-F-Actin(bs-1571R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD  Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (bs-1571R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (bs-1571R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control (blue line): Hela (blue).Primary Antibody (green line): Rabbit Anti- F-Actin antibody (bs-1571R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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