| 中文名稱 | 水通道蛋白4抗體 |
| 別 名 | aquaporin 4; WCH4; Mercurial-insensitive water channel; MIWC; AQP 4; AQP4; Aquaporin4; HMIWC 2; HMIWC2; Mercurial insensitive water channel; MGC22454; MIWC; AQP4_HUMAN; Aquaporin-4; AQP-4. |
| 研究領(lǐng)域 | 腫瘤 信號轉(zhuǎn)導 通道蛋白 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Pig, Cow, Rabbit, Sheep, ) |
| 產(chǎn)品應用 | WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 36kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human AQP4:271-323/323 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. Subunit: Homotetramer. Part of a complex containing MLC1, TRPV4, HEPACAM and ATP1B1. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Brain, muscle, heart, kidney, lung, and trachea. Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family. SWISS: P55088 Gene ID: 361 Database links: Entrez Gene: 281008 Cow Entrez Gene: 361 Human Entrez Gene: 11829 Mouse Entrez Gene: 25293 Rat Omim: 600308 Human SwissProt: O77750 Cow SwissProt: P55087 Human SwissProt: P55088 Mouse SwissProt: P47863 Rat Unigene: 281008 Cow Unigene: 315369 Human Unigene: 250786 Mouse Unigene: 90091 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 通道蛋白（Channel Protein） 水通道蛋白(AQPs)廣泛分布于機體不同組織器官中,腦組織中的水通道蛋白主要為AQP4. AQP-4蛋白主要在星型膠質(zhì)細胞和室管膜細胞內(nèi)表達,尤其在于毛細血管和軟腦膜直接接觸的膠質(zhì)細胞上及血管周表達豐富. 水通道蛋白4的分布特點與其功能密切相關(guān),可能是腦脊液重吸收、滲透壓調(diào)節(jié)、腦水腫形成等生理、病理過程的分子生物學基礎.研究水通道蛋白4可為腦水腫及水代謝疾病提供分子水平的理論依據(jù),同時為臨床治療腦水腫及水代謝疾病提供一種新途徑. |
| 產(chǎn)品圖片 |  Sample: Muscle(Mouse) lysate at 30ug; Primary: Anti-Aquaporin 4 (bs-0634R) at 1:300; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size:36kD Observed band size:35kD  Sample: mouse lung lysate at 30ug;Primary: Anti-Aquaporin 4 (bs-0634R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bse-0295G-HRP) at 1: 5000; Predicted band size:36 kD Observed band size:34 kD  Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated(bs-0634R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Aquaporin 4) Polyclonal Antibody, Unconjugated (bs-0634R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated(bs-0634R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated(bs-0634R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control: K562 (blue).Primary Antibody:Rabbit Anti-Aquaporin 4 antibody (bs-0634R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (bs-0634R, 1μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min at room temperature. Acquisition of 20,000 events was performed.  Blank control:Molt4.Primary Antibody (green line): Rabbit Anti-AQP 4 antibody (bs-0634R-A488) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed. |
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