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中文名稱

整合素αVβ3抗體

別名

CD51 + CD61; CD51; CD61; GP3A; GPIIIa; integrin alpha v; integrin beta 3; intregrin alpha v beta 3; ITGAV; ITGB3; Platelet membrane glycoprotein IIIa; Vitronectin receptor alpha subunit; VNRA; Antigen identified by monoclonal antibody L230; CD51 antigen; CD61 antigen; DKFZp686A08142; Integrin alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); integrin alpha v; Integrin beta 3 (platelet glycoprotein IIIa, antigen CD61); Integrin beta chain beta 3; intregrin alpha v beta 3; ITGAV; MSK8; Platelet glycoprotein IIIa; Platelet membrane glycoprotein IIIa; Vitronectin receptor alpha subunit; Vitronectin receptor subunit alpha; avb3.

研究領(lǐng)域	細(xì)胞生物免疫學(xué) 信號轉(zhuǎn)導(dǎo) 干細(xì)胞細(xì)胞粘附分子
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應(yīng)	Human, Mouse, Rat,
產(chǎn)品應(yīng)用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
細(xì)胞定位	細(xì)胞膜
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human Integrin Alpha V + Beta 3:
亞型	IgG
純化方法	affinity purified by Protein A
儲存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
產(chǎn)品介紹	Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. They are known to participate in cell adhesion as well as cell-surface mediated signalling. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. CD51 encodes integrin alpha chain V. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. The CD61 protein product is the integrin beta chain beta 3. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrin alpha V/beta 3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha V/beta 3 recognizes the sequence R-G-D in a wide array of ligands. The alpha V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. Subcellular Location: Cell Membrane; single-pass type I membrane protein. SWISS: P05106, P06756 Gene ID: 3685 Database links: Entrez Gene: 3685 Human Entrez Gene: 3690 Human Omim: 173470 Human Omim: 193210 Human SwissProt: P05106 Human SwissProt: P06756 Human Unigene: 218040 Human Unigene: 436873 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 整合素αVβ3為二聚體的跨膜糖蛋白質(zhì)，可以調(diào)節(jié)腫瘤細(xì)胞在多種細(xì)胞外基質(zhì)蛋白中的粘附和遷移，在被激活的內(nèi)皮細(xì)胞中有較高的表達(dá)，并在新生血管生成過程中發(fā)揮優(yōu)勢。整合素αVβ3在細(xì)胞外的信號傳入細(xì)胞內(nèi)調(diào)節(jié)細(xì)胞生長、改變細(xì)胞形態(tài)、影響細(xì)胞運(yùn)動, 并在腫瘤侵襲和轉(zhuǎn)移的過程中起重要作用.整合素αVβ3是特異性表達(dá)在血管內(nèi)皮細(xì)胞表面的粘附因子。
產(chǎn)品圖片	Sample: HUVEC (human)Cell Lysate at 40 ug Primary: Anti- Integrin Alpha V + Beta 3 (CD51+CD61) (Ys-1310R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 116 kD Observed band size: 120 kD Tissue/cell: Human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Integrin Alpha V + Beta 3 (CD51 + CD61) Polyclonal Antibody, Unconjugated(Ys-1310R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Integrin Alpha V + Beta 3 (CD51 + CD61) Polyclonal Antibody, Unconjugated(Ys-1310R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining cell: mouse lung. Incubation: Avoid light, at 4°C for 40 minutes. Red line: Blank control (mouse lung cells),2X10^6/ml, at 4°C for 40 minutes. Green line: (primary antibody) Integrin Alpha V + Beta 3 (CD51+CD61) (bs-1310R), (secondary antibody)Goat Anti-rabbit IgG/FITC (bs-0295G-FITC), 1:00, at 4°C for 40 minutes.Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Integrin Alpha V + Beta 3(CD51+CD61) Polyclonal Antibody, Unconjugated(Ys-1310R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: U937(blue). Primary Antibody: Rabbit Anti-Integrin Alpha V + Beta 3 (CD51+CD61) antibody(Ys-1310R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (bs-1310R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.Cell: MCF-7 Concentration:1:100 Host/Isotype:Rabbit/IgG Flow cytometric analysis of Rabbit IgG isotype control (Cat#: Ys-1310R) on MCF-7(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .