| 中文名稱 | 細胞質膜微囊蛋白-1抗體 |
| 別 名 | opamine D3 receptor; Caveolin1; Caveolin 1; 3 dopamine receptor; D; Dopamine receptor D3; DRD; ETM1; FET1; Cav-1; ETM1; CAV 1; CAV1; CAV1_HUMAN. |
| 研究領域 | 信號轉導 細胞凋亡 轉錄調節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1µg/Test IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 20kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Caveolin-1:2-120/178 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene. [provided by RefSeq]. Function: May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Subunit: Homooligomer. Interacts with GLIPR2, NOSTRIN, SNAP25 and syntaxin. Interacts with rotavirus A NSP4. Interacts (via the N-terminus) with DPP4; the interaction is direct. Interacts with CTNNB1, CDH1 and JUP. Interacts with BMX and BTK. Subcellular Location: Golgi apparatus membrane;Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Membrane raft. Note=Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tissue Specificity: Expressed in muscle and lung, less so in liver, brain and kidney. Post-translational modifications: The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress. DISEASE: Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. Similarity: Belongs to the caveolin family. SWISS: Q03135 Gene ID: 857 Database links: Entrez Gene: 403980 Dog Entrez Gene: 857 Human Entrez Gene: 12389 Mouse Entrez Gene: 25404 Rat Omim: 601047 Human SwissProt: P33724 Dog SwissProt: Q03135 Human SwissProt: P49817 Mouse SwissProt: P41350 Rat Unigene: 3850 Dog Unigene: 74034 Human Unigene: 28278 Mouse Unigene: 470537 Mouse Unigene: 22518 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 信號傳導（Signaling Intermediates） Caveolin-1（小凹蛋白）是細胞生長相關信號途徑及腫瘤發(fā)生發(fā)展過程中重要的抑制因子，Caveolae是細胞膜內的特殊膜結構，參與包括細胞的分子運輸、細胞粘附和信號轉導在內的多種細胞活動。Caveolin-1是Caveolae中重要的結構蛋白，抑制細胞生長，與多種人類腫瘤發(fā)生發(fā)展相關的信號分子相互作用。Caveolin在信號轉導的整合中起支架蛋白的作用。Caveolin構成了一個蛋白家族，他們是細胞質膜中發(fā)夾樣結構域的主要結構成分。Caveolin在信號轉導的整合中起支架蛋白的作用。至今已經鑒定了3種Caveolin （Caveolin-1、2 和3），它們具有不同的組織分布。Caveolin-1涉及乳腺上皮細胞增生的發(fā)病機制。該抗體可識別α和β型Caveolin-1。 |
| 產品圖片 |  Sample:Lung (Mouse) Lysate at 40 ug Bladder (Mouse) Lysate at 40 ug Primary: Anti-Caveolin-1 (bs-1453R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kD Observed band size: 20 kD  Sample:Lung (Mouse) Lysate at 40 ug Primary: Anti-Caveolin-1 (bs-1453R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kD Observed band size: 20 kD  Sample:NIH/3T3 Cell Lysate at 40 ug A549 Cell Lysate at 40 ug HUVEC Cell Lysate at 40 ug Primary: Anti-Caveolin-1 (bs-1453R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kD Observed band size: 20 kD  Paraformaldehyde-fixed, paraffin embedded (Rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caveolin-1) Polyclonal Antibody, Unconjugated (bs-1453R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining. Tissue/cell: mouse retina tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Caveolin-1 Polyclonal Antibody, Unconjugated(bs-1453R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Caveolin-1 Polyclonal Antibody, Unconjugated(bs-1453R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green). |
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