| 中文名稱 | 血清應(yīng)答因子抗體 |
| 別 名 | c fos serum response element binding factor; Serum Response Factor SRF; c fos serum response element binding transcription factor; ELK3; ERP; MCM 1; MCM-1; MCM1; OTTHUMP00000039820; SAP2; Serum response factor; SRF; SRF serum response factor c fos serum response element binding transcription factor; SRF_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 干細(xì)胞 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, (predicted: Rat, Dog, Pig, Cow, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 56kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SRF:101-250/508 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). [provided by RefSeq, Jul 2008]. Function: SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation. Subunit: Binds DNA as a multimer, probably a dimer. Interacts with MLLT7/FOXO4, NKX3A and SSRP1. Interacts with ARID2 and SRFBP1. Forms complexes with ARID2, MYOCD, NKX2-5 and SRFBP1. Forms a nuclear ternary complex with MKL1 and SCAI. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated by PRKDC. Similarity: Contains 1 MADS-box domain. SWISS: P11831 Gene ID: 6722 Database links: Entrez Gene: 6722 Human Entrez Gene: 20807 Mouse Entrez Gene: 501099 Rat Omim: 600589 Human SwissProt: P11831 Human SwissProt: Q9JM73 Mouse Unigene: 520140 Human Unigene: 45044 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 轉(zhuǎn)錄調(diào)節(jié)因子（Transcriptin Regulators） 血清應(yīng)答因子(SRF)是一種轉(zhuǎn)錄因子蛋白，可以結(jié)合啟動(dòng)子DNA序列中的血清應(yīng)答元件(SRE)，調(diào)節(jié)一系列基因的表達(dá)，包括即刻早期基因的表達(dá)，如c-fos、fosB、junB、egr-1、egr-2，以及神經(jīng)元基因nurr1、肌肉基因如肌動(dòng)蛋白、肌球蛋白基因等. |
| 產(chǎn)品圖片 |  Sample: NIH/3T3 Cell Lysate at 30 ugPrimary: Anti-SRF (bs-1401R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 56 kD Observed band size: 54 kD  Sample: Muscle(Mouse) Lysate at 30 ugPrimary: Anti- SRF (bs-1401R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 56 kD Observed band size: 56 kD  Sample: Large intestine (Mouse) Lysate at 30 ugPrimary: Anti- SRF (bs-1401R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 56 kD Observed band size: 54 kD  Sample:A431(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug Primary: Anti- SRF (bs-1401R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 52 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SRF) Polyclonal Antibody, Unconjugated (bs-1401R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
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