二抗也稱第二抗體,能和抗體結(jié)合,即抗體的抗體,其主要作用是檢測(cè)抗體的存在,放大一抗的信號(hào),用于靶標(biāo)抗原的間接檢測(cè)。二抗帶有可以被檢測(cè)出的標(biāo)記(如帶熒光、放射性、化學(xué)發(fā)光或顯色基團(tuán)),可以和一抗結(jié)合,通過(guò)信號(hào)放大提高靈敏度,并為標(biāo)記和檢測(cè)提供了更大靈活性?梢哉f(shuō)生物學(xué)研究、醫(yī)藥研究和開(kāi)發(fā)都離不開(kāi)好的二抗產(chǎn)品。
但令很多從事稀有物種研究的學(xué)者頭大的是——選擇一個(gè)物種對(duì)應(yīng)、質(zhì)量又好的二抗產(chǎn)品,真是太難了!猴、沙鼠、水貂、貓,要想生產(chǎn)這些罕見(jiàn)物種的二抗,并不是十分容易,因此鮮少有公司能提供全面的罕見(jiàn)物種靶標(biāo)二抗。
不過(guò)!看過(guò)小B這篇文章之后,一切問(wèn)題都迎刃而解啦!提供品類豐富的高質(zhì)量標(biāo)記和未標(biāo)記二抗,涵蓋多種稀有罕見(jiàn)物種;可用于一抗的熒光、顯色和化學(xué)發(fā)光檢測(cè),適合各種應(yīng)用,例如蛋白質(zhì)免疫印跡、免疫組化、流式細(xì)胞檢測(cè)和細(xì)胞成像。
二抗靶標(biāo)物種
常見(jiàn)物種:兔,小鼠,大鼠,羊,人
稀有物種:豚鼠,雞,狗,豬,馬,牛
罕見(jiàn)物種:猴,長(zhǎng)爪沙鼠,水貂,貓
英文名稱 | Rabbit Anti-dog IgG |
中文名稱 | 兔抗狗IgG |
別 名 | Immunoglobulin G |
抗體來(lái)源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | dog |
產(chǎn)品應(yīng)用 | WB=1:1000-5000 ELISA=1:2000-10000 IHC-P=1:200-1000 IHC-F=1:200-1000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 150kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 10mg/1ml |
免 疫 原 | Full length plasma protein:full length |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲(chǔ) 存 液 | 0.01M PBS(pH7.4) |
保存條件 | Storage: Store at –20 oC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20oC. When reconstituted in sterile distilled water or diluent supplied, theantibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |