| 中文名稱 | 骨/軟骨蛋白多糖1抗體 |
| 別 名 | BGN; Biglycan; Biglycan proteoglycan; Bone/cartilage proteoglycan I; Dermatan sulphate proteoglycan I; DSPG1; PG S1; PG-S1; PGI; PGS1_HUMAN; SLRR1A; Small leucine rich protein 1A. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 干細(xì)胞 細(xì)胞骨架 細(xì)胞外基質(zhì) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 37kDa |
| 細(xì)胞定位 | 細(xì)胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Biglycan:101-200/368 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | May be involved in collagen fiber assembly. Tissue specificity; Found in several connective tissues, especially in articular cartilages. Function: May be involved in collagen fiber assembly (By similarity). Subunit: Homodimer. Forms a ternary complex with MFAP2 and ELN Subcellular Location: Secreted, extracellular space, extracellular. Tissue Specificity: Found in several connective tissues, especially in articular cartilages. Post-translational modifications: The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate (By similarity). Similarity: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. Contains 12 LRR (leucine-rich) repeats. SWISS: P21810 Gene ID: 633 Database links: Entrez Gene: 633 Human Entrez Gene: 12111 Mouse Entrez Gene: 25181 Rat Omim: 301870 Human SwissProt: P21810 Human SwissProt: P28653 Mouse SwissProt: P47853 Rat Unigene: 821 Human Unigene: 2608 Mouse Unigene: 783 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |  Sample: Bone (mouse) Lysate at 40 ugPrimary: Anti- Biglycan (bs-7552R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37kD Observed band size: 52 kD  Sample:Colon (Rat) Lysate at 40 ug Embryo (Mouse) Lysate at 40 ug Primary: Anti-Biglycan (bs-7552R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 37 kD Observed band size: 52 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Biglycan) Polyclonal Antibody, Unconjugated (bs-7552R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Biglycan Polyclonal Antibody, Unconjugated(bs-7552R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: human endothelial cells;4% Paraformaldehyde-fixed;Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Biglycan Polyclonal Antibody, Unconjugated(bs-7552R) 1:50, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Rhodamine B conjugated (bs-0295G-RBITC) used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei |
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