| 中文名稱 | 白介素1β抗體 |
| 別 名 | Catabolin; H1; IL-1β; Hematopoietin 1; IFN beta inducing factor; IL 1; IL 1 beta; IL 1B; IL1B; IL1F2; Interleukin 1 beta; Interleukin 1 beta precursor; LAF; OAF; Osteoclast activating factor; Preinterleukin beta; Pro interleukin 1 beta; IL1B_HUMAN; Interleukin-1 beta; IL-1 beta. |
| 研究領(lǐng)域 | 細胞生物 免疫學 生長因子和激素 糖蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse, Rat, (predicted: Human, Dog, Horse, Rabbit, ) |
| 產(chǎn)品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 17/32kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IL-1 Beta:161-269/269 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]. Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Subunit: Monomer. Subcellular Location: Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. Similarity: Belongs to the IL-1 family. SWISS: P01584 Gene ID: 3553 Database links: Entrez Gene: 3553 Human Entrez Gene: 16176 Mouse Entrez Gene: 24494 Rat Omim: 147720 Human SwissProt: P01584 Human SwissProt: P10749 Mouse SwissProt: Q63264 Rat Unigene: 126256 Human Unigene: 222830 Mouse Unigene: 9869 Rat
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |  Sample:Raw264.7(Mouse) Cell Lysate at 30 ug Liver (Mouse) Lysate at 40 ug Primary: Anti-IL-1 Beta (bs-6319R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/30 kD Observed band size: 30 kD  Sample:Brain (Mouse) Lysate at 40 ug Intestine (Mouse) Lysate at 40 ug Primary: Anti-IL-1 Beta (bs-6319R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 17/30 kD Observed band size: 30 kD  Sample:Raw264.7(Mouse) Cell Lysate at 30 ug Primary: Anti- IL-1 Beta (bs-6319R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/30 kD Observed band size: 30 kD  Sample:uterus (Mouse) Lysate at 40 ug Primary: Anti-IL-1 Beta (Bs- 6319R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/30 kD Observed band size: 27 kD  Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-1 Beta) Polyclonal Antibody, Unconjugated (bs-6319R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
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