| 中文名稱 | 肽基脯氨酰順反異構(gòu)酶FKBP10抗體 |
| 別 名 | 65 kDa FK506 binding protein; FK506 binding protein 10 65 kDa; FK506 binding protein 10; FKBP 10; FKBP 65; hFKBP 65; Immunophilin FKBP65; Peptidyl prolyl cis trans isomerase. PPIase; Rotamase; FKB10_HUMAN. |
| 研究領域 | 細胞生物 信號轉(zhuǎn)導 轉(zhuǎn)運蛋白 結(jié)合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Sheep, ) |
| 產(chǎn)品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 61kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FKBP10:31-130/582 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | The immunophilins are a highly conserved family of cis-trans peptidyl-prolyl isomerases that bind to and mediate the effects of immunosuppressive drugs, such as cyclosporin, FK506 and rapamycin. Immunophilins have also been implicated in protein folding and trafficking within the endoplasmic reticulum (ER). FKBP10 (FK506-binding protein 10), also known as peptidyl-prolyl cis-trans isomerase, PPIase, Rotamase, 65kDa FK506-binding protein or FKBP65, is a 582 amino acid immunophilin localized to the ER lumen and found in many tissues including heart, spleen, brain, testis and lung. FKBP10 contains two EF-hand calcium-binding domains and four PPIase FKBP-type domains, suggesting an enzymatic role in protein folding by catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. FKBP10 also acts as a receptor for the immunosuppressants FK506 and rapamycin, which inhibit FKBP10 activity. FKBP10 is thought to interact with the Raf-1/HSP 90 heterocomplex during signal transduction processes, and may associate with elastin during elastin protein folding and transport. With a valine-24 addition to human FKBP10, human and mouse FKBP10 are almost identical. Function: FKBP10 (FK506 binding protein 10) belongs to the FKBP type peptidyl-prolyl cis/trans isomerase (PPIase) family. PPIases accelerate the folding of proteins during protein synthesis, acting as molecular chaperones. FKBP10 is located in the endoplasmic reticulum (ER) and is thought to play a role in the folding and trafficking of secretory proteins. Subcellular Location: Endoplasmic reticulum. Post-translational modifications: Glycosylated and phosphorylated. DISEASE: Defects in FKBP10 are the cause of osteogenesis imperfecta type 6 (OI6) [MIM:610968]. OI6 is a moderate to severe, autosomal recessive form of osteogenesis imperfecta characterized by increased serum alkaline phosphatase levels and bone histology exhibiting a fish scale-like lamellar pattern. Osteogenesis imperfecta defines a group of connective tissue disorders characterized by bone fragility and low bone mass. Similarity: Contains 2 EF-hand domains. Contains 4 PPIase FKBP-type domains. SWISS: Q96AY3 Gene ID: 60681 Database links: Entrez Gene: 60681 Human Omim: 607063 Human SwissProt: Q96AY3 Human Unigene: 463035 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |  Sample:293T Cell(Human)Lysate at 40 ugPrimary: Anti-FKBP10(bs-13175R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61kD Observed band size: 61kD  Sample: Uterus (Mouse) Lysate at 40 ugPrimary: Anti-FKBP10(bs-13175R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 76 kD  Sample: MCF-7(human) Cell Lysate at 40 ugPrimary: Anti-FKBP10(bs-13175R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 61 kD  Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FKBP10) Polyclonal Antibody, Unconjugated (bs-13175R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FKBP10) Polyclonal Antibody, Unconjugated (bs-13175R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
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