| 中文名稱 | 瘦素受體抗體 |
| 別 名 | obl; CD 295; CD295; CD295 antigen; Db; Fa; HuB219; LEP R; LEPR; LEPROT; Leptin receptor fatty; Leptin receptor gene related protein; Leptin receptor precursor; OB R; OB-R; OB R gene related protein; OB receptor; OB-RGRP; Obr; Ob-R a/b/c/d/e; LEPR_HUMAN; LEPRD; Leptin receptor. |
| 研究領(lǐng)域 | 心血管 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 生長因子和激素 脂蛋白 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, (predicted: Rat, Dog, Pig, Horse, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 103/132kDa |
| 細(xì)胞定位 | 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Leptin receptor:251-350/1165 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010] Function: Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway (By similarity). Subunit: On leptin stimulation, homodimerizes. The phosphorylated receptor binds a number of SH2 domain-containing proteins such as STAT3, PTPN11, and SOCS3. Interaction with SOCS3 inhibits LRb signaling. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Isoform E: Secreted (Probable). Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus. Tissue Specificity: Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus. Post-translational modifications: On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similarity). Phosphorylation on Tyr-1141 is required for STAT3 binding/activation. Similarity: Belongs to the type I cytokine receptor family. Type 2 subfamily. Contains 4 fibronectin type-III domains. Contains 1 Ig-like (immunoglobulin-like) domain. SWISS: P48357 Gene ID: 3953 Database links: Entrez Gene: 3953 Human Entrez Gene: 16847 Mouse Entrez Gene: 24536 Rat Omim: 601007 Human SwissProt: P48357 Human SwissProt: P48356 Mouse SwissProt: Q62959 Rat Unigene: 23581 Human Unigene: 723178 Human Unigene: 259282 Mouse Unigene: 9891 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Leptin是一種分子量為16kDa的脂肪組織源激素，由脂肪細(xì)胞分泌的，具有強親水性，以單體形式存在于血漿中。 Leptin 具有廣泛的生物學(xué)效應(yīng)，作用于下丘腦，調(diào)節(jié)食欲、能量代謝及體重。Leptin 還可能作為脂肪-胰島內(nèi)分泌軸一部分，參與胰島素分泌的調(diào)節(jié)。Leptin receptor (OB-R)是與gp130最密切相關(guān)的信號轉(zhuǎn)導(dǎo)的共同成分,屬1類細(xì)胞因子超家族，下丘腦有豐富的Leptin receptor，在其它部位也有分布，但水平較低：如胰島素B細(xì)胞；脈絡(luò)叢；肝、肺、心、腎、睪丸、淋巴細(xì)胞和脂肪細(xì)胞中。 人類存在四個異性體，均為單跨膜受體。其中OB-Rb在胞內(nèi)結(jié)構(gòu)域最長，是唯一能進行信號傳導(dǎo)并調(diào)節(jié)熱量攝入和能量消耗的異性體,分子量為132kDa。 |
| 產(chǎn)品圖片 |  Sample:HepG2(Human) Cell Lysate at 30 ug Primary: Anti-Leptin receptor (bs-20498R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 103/132 kD Observed band size: 132 kD  Sample:Huvec(Human) Cell Lysate at 30 ug Primary: Anti-Leptin receptor (bs-20498R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 103/132 kD Observed band size: 132 kD  Sample:Lane 1: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 2: Hela (Human) Cell Lysate at 30 ug Lane 3: K562 (Human) Cell Lysate at 30 ug Primary: Anti-Leptin receptor (bs-20498R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 103/132 kD Observed band size: 120 kD  Sample:Lung (Mouse) Lysate at 40 ug Primary: Anti-Leptin receptor (bs-20498R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 103/132 kD Observed band size: 132 kD  Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Leptin receptor) Polyclonal Antibody, Unconjugated (bs-20498R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
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