| 中文名稱 | 神經(jīng)生長因子受體抗體 |
| 別 名 | NGFR; Gp80 LNGFR; ngfrp75; Low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; NGF receptor; p75 ICD; CD271; CD271 antigen; Gp80 LNGFR; Low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; Nerve growth factor receptor; Nerve growth factor receptor TNFR superfamily member 16; NGF receptor; NGFR; p75 ICD; p75 Neurotrophin receptor; p75 NTR; TNFR Superfamily Member 16; TNFRSF16; Tumor necrosis factor receptor superfamily member 16; TNR16_HUMAN; NGFR p75. |
| 研究領域 | 細胞生物 神經(jīng)生物學 信號轉(zhuǎn)導 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Horse, Guinea Pig, ) |
| 產(chǎn)品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 44kDa |
| 細胞定位 | 細胞核 細胞漿 細胞膜 細胞外基質(zhì) |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NGFR:301-400/427 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | The low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells. NGF is important for the development, differentiation and survival of a variety of neuronal and non-neuronal cells. Its action is mediated by binding to two distinct receptors, the high affinity p140 and low affinity p75. p75NGFR binds neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, and NT-6. p75NGFR belongs to the TNF-R superfamily and is reported to mediate NGF-induced apoptosis. Function: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Subunit: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1. Interacts with KIDINS220 and NTRK1. Can form a ternary complex with NTRK1 and KIDINS220 and this complex is affected by the expression levels of KIDINS220. An increase in KIDINS220 expression leads to a decreased association of NGFR and NTRK1. Interacts with LINGO1 (By similarity). Interacts with NTRK2; may regulate the ligand specificity of the NTRK2 receptor. Interacts with NRADD. Subcellular Location: Membrane; Single-pass type I membrane protein. Post-translational modifications: N- and O-glycosylated. Phosphorylated on serine residues. Similarity: Contains 1 death domain. Contains 4 TNFR-Cys repeats. SWISS: P07174 Gene ID: 4804 Database links: Entrez Gene: 4804 Human Entrez Gene: 18053 Mouse Entrez Gene: 24596 Rat Omim: 162010 Human SwissProt: P08138 Human SwissProt: Q9Z0W1 Mouse SwissProt: P07174 Rat Unigene: 415768 Human Unigene: 681726 Human Unigene: 283893 Mouse Unigene: 10980 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 神經(jīng)生物學相關蛋白（Neurobiology） NGFR神經(jīng)生長因子受體又稱：CD271存在與細胞上的與神經(jīng)生長因子相結(jié)合并相互作用的蛋白質(zhì)因子，屬一型跨膜TNF受體超家族糖蛋白，它可與NGF, BDNF, NT-3, NT-4 結(jié)合，根據(jù)與神經(jīng)生長因子的結(jié)合力速度及親和力可分為高親和力和低親和力兩種NGFR。 此抗體識別分子量為47kDa低親和力的NGFR.NGF-R也稱為低親和神經(jīng)生長因子受體，屬1型跨膜TNF受體超家族。 |
| 產(chǎn)品圖片 |  Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NGF Receptor) polyclonal Antibody, Unconjugated (bs-0161R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control (blue line): Mouse spleen (blue).Primary Antibody (green line): Rabbit Anti- p75 NGF Receptor antibody (bs-0161R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 70% ice-cold methanol overnight at 4℃ and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.). Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.  Blank control:Mouse brain.Primary Antibody (green line): Rabbit Anti-p75 NGF Receptor antibody (bs-0161R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.  Blank control: (mo)nephrocyte(blue)Isotype Control Antibody: Rabbit IgG -AF647(orange); Primary Antibody Dilution: 5μl in 100 μL1X PBS containing 0.5% BSA(green). |
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