| 中文名稱 | 陰離子轉(zhuǎn)運(yùn)蛋白-1抗體 |
| 別 名 | Organic anion transporter 1; OAT1; OAT-1; solute carrier family 22 (organic anion transporter), member 6; FLJ55736; HOAT1; MGC124962; MGC45260; mOat1; NKT; Oat; Orctl1; PAHT; ROAT1; SLC22A6; S22A6_HUMAN. |
| 研究領(lǐng)域 | 轉(zhuǎn)運(yùn)蛋白 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 62kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human OCT-1:285-550/550 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Recent advances in molecular biology have identified three organic anion transporter families: the organic anion transporter (OAT) family encoded by SLC22A, the organic anion transporting peptide (OATP) family encoded by SLC21A (SLCO), and the multidrug resistance-associated protein (MRP) family encoded by ABCC. These families play critical roles in the transepithelial transport of organic anions in the kidneys as well as in other tissues such as the liver and brain. Among these families, the OAT family plays the central role in renal organic anion transport. Knowledge of these three families at the molecular level, such as substrate selectivity, tissue distribution, and gene localization, is rapidly increasing. Function: Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of 2,3-dimercapto-1-propanesulfonic acid (DMPS) (By similarity). Mediates the sodium-independent uptake of p-aminohippurate (PAH), ochratoxin (OTA), acyclovir (ACV), 3'-azido-3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF), cidofovir, adefovir, 9-(2-phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl) diaminopurine (PMEDAP) and edaravone sulfate. PAH uptake is inhibited by p-chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), sulindac, diclofenac, carprofen, glutarate and okadaic acid (By similarity). PAH uptake is inhibited by benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, furosemide, indomethacin, bumetamide, losartan, probenecid, phenol red, urate, and alpha-ketoglutarate. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Strongly expressed in kidney and to a lower extent in liver, skeletal muscle, brain and placenta. Found at the basolateral membrane of the proximal tubule. Post-translational modifications: Glycosylated. Glycosylation at Asn-113 may occur at a secondary level. Glycosylation is necesssary for proper targeting of the transporter to the plasma membrane. Similarity: Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. SWISS: Q4U2R8 Gene ID: 9356 Database links: Entrez Gene: 9356 Human Entrez Gene: 18399 Mouse Entrez Gene: 29509 Rat Omim: 607582 Human SwissProt: Q4U2R8 Human SwissProt: Q8VC69 Mouse SwissProt: O35956 Rat Unigene: 369252 Human Unigene: 30090 Mouse Unigene: 87849 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. OAT1 陰離子轉(zhuǎn)運(yùn)蛋白是一類分布廣泛的膜蛋白，通過(guò)介導(dǎo)Cl－／HCO－3跨膜轉(zhuǎn)運(yùn)參與細(xì)胞內(nèi)pH、細(xì)胞體積及細(xì)胞內(nèi)氯離子濃度的調(diào)節(jié)。bs-0607R為抗大、小鼠。 |
| 產(chǎn)品圖片 |  Sample: Raji Cell lysate at 30 ug;Primary:Anti-SLC22A6 (bs-0606R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size: 62 kD Observed band size: 62 kD  Sample:Kidney(Human) lysate at 30ug; Brain(Rat) lysate at 30ug; Primary: Anti-OAT-1/SLC22A6(human) (bs-0606R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 62kD Observed band size : 61kD  Sample:Liver (Mouse) Lysate at 40 ug Primary: Anti-SLC22A6 (bs-0606R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 62 kD  Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-OAT-1/SLC22A6(human) Polyclonal Antibody, Unconjugated(bs-0606R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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