| 中文名稱 | 基質(zhì)細(xì)胞衍生因子－1抗體 |
| 別 名 | Chemokine (C X C motif) ligand 12; Chemokine (CXC motif) ligand 12; CXCL 12; CXCL12; hIRH; IRH; PBSF; Pre B cell growth stimulating factor; SCYB 12; SCYB12; SDF 1; SDF 1 beta; SDF 1a; SDF 1b; SDF1; SDF1a; SDF1b; Stromal cell derived factor 1; Stromal cell derived factor 1 beta; TLSF a; TLSF; TLSF b; TLSFa; TLSFb; TPAR 1; TPAR1. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 趨化因子 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Rat, (predicted: Mouse, Chicken, Dog, Rabbit, ) |
| 產(chǎn)品應(yīng)用 | ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 8kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SDF-1:21-93/93 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | This gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]. Function: Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to another C-X-C chemokine receptor CXCR7, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and CXCR7, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of CXCR7 expressed in various cells. Subunit: Monomer or homodimer; in equilibrium. Dimer formation is induced by non acidic pH and the presence of multivalent anions, and by binding to CXCR4 or heparin. Monomeric form is required for full chemotactic activity and resistance to ischemia/reperfusion injury, whereas the dimeric form acts as a partial agonist of CXCR4, stimulating Ca2+ mobilization but with no chemotactic activity and instead acts as a selective antagonist that blocks chemotaxis induced by the monomeric form. Interacts with the N-terminus of CXCR7. Subcellular Location: Secreted. Tissue Specificity: Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen. Post-translational modifications: Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans. Similarity: Belongs to the intercrine alpha (chemokine CxC) family. SWISS: P48061 Gene ID: 6387 Database links: Entrez Gene: 6387 Human Entrez Gene: 20315 Mouse Entrez Gene: 24772 Rat SwissProt: P48061 Human SwissProt: P40224 Mouse Omim: 600835 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 基質(zhì)細(xì)胞衍生因子－1(SDF-1)又稱趨化因子CXCL12 是小分子的細(xì)胞因子，屬于趨化因子蛋白家族。它有兩種形式, SDF-1α/CXCL12a和SDF-1β/CXCL12b。趨化因子有四個(gè)保守的半胱氨酸殘基形成兩對(duì)雙硫鍵以構(gòu)成趨化因子的特殊結(jié)構(gòu)。第一第二半胱氨酸殘基之間隔著一個(gè)介入氨基酸殘基。 基質(zhì)細(xì)胞源性生長(zhǎng)因子1:趨化因子CXCL12對(duì)淋巴細(xì)胞有強(qiáng)烈的趨化作用并在發(fā)育中起重要作用。在胚胎發(fā)育中CXCL12引導(dǎo)造血干細(xì)胞從胎兒肝臟到骨髓的遷徙。 CXCL12可以在許多組織(包括腦,胸腺,心,肺,肝,腎,骨髓,脾臟)中表達(dá)。 趨化因子CXCL12的受體是CXCR4。但是,最近有人認(rèn)為CXCL12還可以與CXCR7受體結(jié)合。 |
| 產(chǎn)品圖片 |  Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SDF1 beta) Polyclonal Antibody, Unconjugated (bs-0783R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: Rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SDF1 beta Polyclonal Antibody, Unconjugated(bs-0783R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: human hepatocellular carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SDF1 beta Polyclonal Antibody, Unconjugated(bs-0783R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SDF-1/CXCL12 Polyclonal Antibody, Unconjugated(bs-0783R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
我要詢價(jià)
*聯(lián)系方式：
(可以是QQ、MSN、電子郵箱、電話等，您的聯(lián)系方式不會(huì)被公開)
*內(nèi)容：
