中文名稱 組織蛋白酶L抗體
別 名 Cathepsin L heavy chain; Cathepsin L light chain; CathepsinL; CATL; CTSL; CTSL protein; DKFZp686A18159; FLJ31037; Major excreted protein; MEP; cath-L; CL; CATL1_HUMAN; CathepsinL1; Cathepsin L1,Cathepsin-L1; CTSL 1; CTSL-1,CTS L1,CTS-L1.
研究領(lǐng)域 激酶和磷酸酶
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, Rat, (predicted: Mouse, )
產(chǎn)品應(yīng)用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 19/30/37kDa
細胞定位 細胞漿
性 狀 Liquid
濃 度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human cathepsin L1 proprotein:71-170/334
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene.
Function:
Important for the overall degradation of proteins in lysosomes.
Subunit:
Dimer of a heavy and a light chain linked by disulfide bonds.
Subcellular Location:
Lysosome.
Similarity:
Belongs to the peptidase C1 family.
SWISS:
P06797
Gene ID:
1514
Database links:
Entrez Gene: 1514 HumanEntrez Gene: 13039 MouseEntrez Gene: 25697 RatOmim: 116880 HumanSwissProt: P07711 HumanSwissProt: P06797 MouseSwissProt: P07154 RatUnigene: 726015 HumanUnigene: 930 MouseUnigene: 1294 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
合成與降解（Synthesi組織蛋白酶L(cathepsin L CL)屬于木瓜蛋白酶家族中的半胱氨酸蛋白水解酶，以酶原的形式貯存于溶酶體中. 參與許多特殊的生理過程，如激素原的激活、抗原呈遞、組織器官的發(fā)育等。
在病理狀態(tài)下，因各種原因所致的細胞損傷(如病原微生物、炎癥因子、氧化應(yīng)激等)可降解細胞成分或細胞間質(zhì)基質(zhì)成分，包括層粘素、IV型膠原纖維及纖維連接素等，與人類許多疾病如腫瘤的浸潤與轉(zhuǎn)移、關(guān)節(jié)炎、骨質(zhì)疏松、阿爾茨海姆病、多發(fā)性硬化癥及其他慢性炎癥性疾病有關(guān).
| 產(chǎn)品圖片 |  Sample: A549 Cell (Human) Lysate at 30 ug K562 Cell (Human) Lysate at 30 ug Primary: Anti- Cathepsin L (bs-1508R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19/30/37 kD Observed band size: 40 kD  Protein: line1, rat brain lysates, 30ug; line2, rat liver lysates, 30ug; Primary: Anti-Cathepsin L (bs-1508R) at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 37kD Observed band size : 19kD, 37kD  Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cathepsin Polyclonal Antibody, Unconjugated(bs-1508R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cathepsin L Polyclonal Antibody, Unconjugated(bs-1508R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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