| 中文名稱 | 白介素-22抗體 |
| 別 名 | Interleukin-22; Il22; IL-22; Iltif; ILTIFa; MGC129416; MGC129417; IL-10-related T-cell-derived-inducible factor; IL-TIF; IL-TIF alpha; Interleukin-22a; IL-22a; IL 21; IL D110; IL TIF; IL21; IL22; ILD110; interleukin 21; MGC79382; MGC79384; TIFa; TIFIL 23; TIFIL23; zcyto18. |
| 研究領(lǐng)域 | 免疫學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, (predicted: Rat, Dog, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 17kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IL-22:35-179/179 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | This gene was identified by its overexpression specifically in herpesvirus samimiri-transformed T cells. The encoded protein is a member of the IL10 family of cytokines. It is a secreted protein and may function as a homodimer. This protein is thought to contribute to the transformed phenotype of T cells after infection by herpesvirus samimiri. [provided by RefSeq]. Function: Cytokine that contributes to the inflammatory response in vivo. Subcellular Location: Secreted. Similarity: Belongs to the IL-10 family. SWISS: Q9GZX6 Gene ID: 50616 Database links: Entrez Gene: 50616 Human Entrez Gene: 50929 Mouse Entrez Gene: 500836 Rat Omim: 605330 Human SwissProt: Q9GZX6 Human SwissProt: Q9JJY9 Mouse Unigene: 287369 Human Unigene: 103585 Mouse Unigene: 195879 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. IL-22是白介素-10家族的成員,主要是由激活的T 淋巴細(xì)胞產(chǎn)生的一類重要的細(xì)胞因子,除了在Th17細(xì)胞中高表達(dá)之外，在NK和NKT等細(xì)胞中也有表達(dá),在組織損傷修復(fù)以及牛皮癬、肝炎、肺炎、腸炎等慢性炎癥中發(fā)揮重要作用.IL-22是繼IL-6和IL-10之后又一個(gè)參與炎癥反應(yīng)和代謝調(diào)控的細(xì)胞因子，IL-22有可能成為干預(yù)脂肪肝發(fā)生發(fā)展的新方法。 |
| 產(chǎn)品圖片 |  Sample:Lane 1: 293T (Human) Cell Lysate at 30 ug Lane 2: A549 (Human) Cell Lysate at 30 ug Primary: Anti-IL22 (bs-2623R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kD Observed band size: 18 kD  Sample:A549(Human) Cell Lysate at 30 ug Primary: Anti-IL22 (bs-2623R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 19 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL22) Polyclonal Antibody, Unconjugated (bs-2623R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining. Blank control(blue): Mouse liver cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).Primary Antibody: Rabbit Anti-IL22 antibody(bs-2623R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. |
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