| 中文名稱 | 肺Kruppel樣因子抗體 |
| 別 名 | Kruppel like factor 2; Kruppel like factor; Kruppel like factor LKLF; Kruppel-like factor 2 (lung); Lung krueppel like factor; Lung Kruppel like zinc finger transcription factor; KLF 2; Klf2; KLF2_HUMAN; Krueppel-like factor 2; Lklf; Lung krueppel like factor; Lung krueppel-like factor; Lung Kruppel like zinc finger transcription factor. |
| 研究領域 | 腫瘤 細胞生物 信號轉導 轉錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Pig, Cow, Horse, Rabbit, Sheep, ) |
| 產(chǎn)品應用 | WB=1:100-500 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:ug/Test IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 37kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KLF2:251-355/355 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Kruppel-like factors (KLFs) are a family of broadly expressed zinc finger transcription factors. KLF2 regulates T-cell trafficking by promoting expression of the lipid-binding receptor S1P1 (S1PR1; MIM 601974) and the selectin CD62L (SELL; MIM 153240) (summary by Weinreich et al., 2009 [PubMed 19592277]).[supplied by OMIM, Feb 2011] Function: Binds to the CACCC box in the beta-globin gene promoter and activates transcription . Subunit: Interacts with WWP1. Subcellular Location: Nucleus. Post-translational modifications: Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein. Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers. SWISS: Q9Y5W3 Gene ID: 10365 Database links: Entrez Gene: 10365 Human Entrez Gene: 16598 Mouse Entrez Gene: 306330 Rat Omim: 602016 Human SwissProt: Q9Y5W3 Human SwissProt: Q60843 Mouse SwissProt: Q9ET58 Rat Unigene: 107740 Human Unigene: 726356 Human Unigene: 26938 Mouse Unigene: 92653 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |  Sample:Lane1:Brain (Mouse) Lysate at 30 ug Lane2:HepG2 Cell Lysate at 30 ug Primary: Anti-KLF2 (bs-2772R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution; Predicted band size :39 kD Observed band size :39 kD  Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (KLF2) Polyclonal Antibody, Unconjugated (bs-2772R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Tissue/cell: Mouse embryos;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer (0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation:Anti-KLF2 Polyclonal Antibody, Unconjugated(bs-2772R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei  Blank control: A431.Primary Antibody (green line): Rabbit Anti-KLF2 antibody (bs-2772R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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