Entrez Gene: 4634 Human Entrez Gene: 17897 Mouse Entrez Gene: 24585 Rat Omim: 160790 Human SwissProt: P08590 Human SwissProt: P09542 Mouse SwissProt: P16409 Rat Unigene: 517939 Human Unigene: 7353 Mouse Unigene: 1955 Rat
中文名稱 肌球蛋白輕鏈3抗體 別 名 Cardiac myosin light chain 1; Slow skeletal Myosin; CMH8; CMLC1; MLC1SB; MLC1V; MYL3; MYL3_HUMAN; Myosin light chain 1; Myosin light chain 1 slow twitch muscle B ventricular isoform; myosin light chain 3 alkali ventricular skeletal slow; Myosin light chain 3; slow-twitch muscle B/ventricular isoform; Ventricular slow twitch myosin alkali light chain; Ventricular/slow twitch myosin alkali light chain; VLC1. 研究領(lǐng)域 心血管 免疫學(xué) 抗體來(lái)源 Rabbit 克隆類型 Polyclonal 交叉反應(yīng) Mouse, (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Rabbit, ) 產(chǎn)品應(yīng)用 WB=1:500-2000 ELISA=1:1000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user. 分 子 量 22kDa 細(xì)胞定位 細(xì)胞漿 性 狀 Liquid 濃 度 1mg/ml 免 疫 原 KLH conjugated synthetic peptide derived from human MLC/Myosin light chain 3:101-195/195 亞 型 IgG 純化方法 affinity purified by Protein A 儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. PubMed PubMed 產(chǎn)品介紹 Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. Human myosin light chain has clinical application as a cardiac marker.
Function:
Regulatory light chain of myosin. Does not bind calcium.
Subunit:
Myosin is a hexamer of 2 heavy chains and 4 light chains.
Post-translational modifications:
The N-terminus is blocked.
N-terminus is methylated by METTL11A/NTM1.
DISEASE:
Cardiomyopathy, familial hypertrophic 8 (CMH8)[MIM:608751]: A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Rarely, patients present a variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening. Note=The disease is caused by mutations affecting the gene represented in this entry.
Similarity:
Contains 3 EF-hand domains.
SWISS:
P08590
Gene ID:
4634
Database links:
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品圖片 
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-MYL3 (bs-2240R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD
Sample: Placenta (Mouse) Lysate at 30 ug
Primary: Anti- MYL3(bs-2240R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 22kD
Observed band size: 22 kD
Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MYL3 Polyclonal Antibody, Unconjugated(bs-2240R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MYL3 Polyclonal Antibody, Unconjugated(bs-2240R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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