Entrez Gene: 83933 Human Entrez Gene: 170787 Mouse Entrez Gene: 362981 Rat Omim: 608544 Human SwissProt: Q969S8 Human SwissProt: Q6P3E7 Mouse SwissProt: Q569C4 Rat Unigene: 26593 Human Unigene: 203954 Mouse Unigene: 107028 Rat
中文名稱 組蛋白去乙�；�10抗體 別 名 HD 10; HD10; HDAC 10; Histone deacetylase 10; MGC149722; OTTHUMP00000028555; DKFZP761B039; HDA10_HUMAN; Histone deacetylase 10; HD10. 研究領(lǐng)域 腫瘤 免疫學(xué) 發(fā)育生物學(xué) 信號轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 抗體來源 Rabbit 克隆類型 Polyclonal 交叉反應(yīng) Human, Mouse, Rat, (predicted: Pig, Cow, Horse, Rabbit, ) 產(chǎn)品應(yīng)用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user. 分 子 量 71kDa 細(xì)胞定位 細(xì)胞核 細(xì)胞漿 性 狀 Liquid 濃 度 1mg/ml 免 疫 原 KLH conjugated synthetic peptide derived from human HDAC10:561-669/669 亞 型 IgG 純化方法 affinity purified by Protein A 儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. PubMed PubMed 產(chǎn)品介紹 The enzymes responsible for the reversible acetylation/ deacetylation process of histones are histone acetyltransferases (HATs) and histone deacetylases (HDACs), respectively. HATs act as transcriptional coactivators and HDACs are part of transcriptional corepressor complexes. Mammalian HDACs can be divided into three classes according to sequence homology. Class I consists of the yeast Rpd3 like proteins HDAC1, HDAC2, HDAC3, and HDAC8. Class II consists of the yeast Hda1 like proteins HDAC10, HDAC5, HDAC6, HDAC7, HDAC9, and HDAC10. Class III comprises the yeast Sir2 like proteins. Whereas class I HDACs are ubiquitously expressed, most class II HDACs are tissue specific. HDAC10 is similar to HDAC6, both containing a unique putative second catalytic domain not found in other HDACs. However, this domain is not functional in HDAC10. The deacetylase activity of class II HDACs is regulated by subcellular localization. HDAC10 was localized to both the nucleus and cytoplasm. HDAC10 can deacetylate histones, repress transcription, and interact with HDAC3.
Function:
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Subunit:
Interacts with HDAC2, HDAC3 and NCOR2.
Subcellular Location:
Cytoplasm. Nucleus. Note=Excluded from the nucleoli.
Tissue Specificity:
Ubiquitous. High expression in liver, spleen, pancreas and kidney.
Similarity:
Belongs to the histone deacetylase family. HD type 2 subfamily.
SWISS:
Q969S8
Gene ID:
83933
Database links:
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品圖片 
Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HDAC10) Polyclonal Antibody, Unconjugated (bs-2893R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HDAC10) Polyclonal Antibody, Unconjugated (bs-2893R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HDAC10) Polyclonal Antibody, Unconjugated (bs-2893R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HDAC10) Polyclonal Antibody, Unconjugated (bs-2893R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
