Entrez Gene: 477753 Dog Entrez Gene: 240 Human Entrez Gene: 11689 Mouse Entrez Gene: 25290 Rat Omim: 152390 Human SwissProt: P09917 Human SwissProt: P48999 Mouse SwissProt: P12527 Rat Unigene: 89499 Human Unigene: 41072 Mouse Unigene: 9662 Rat
中文名稱 磷酸化5-脂氧合酶抗體 別 名 5-Lipoxygenase (Phospho Ser663); 5-Lipoxygenase (Phospho S663); 5-lipoxygenase; 5 LO; 5LPG; ALOX 5; ALOX5; Arachidonate 5 lipoxygenase; Arachidonic acid 5 lipoxygenase; Leukotriene A4 synthase; LOG 5; LOG5; MGC163204; LOX5_HUMAN. 產品類型 磷酸化抗體 研究領域 腫瘤 免疫學 信號轉導 轉錄調節(jié)因子 激酶和磷酸酶 合成與降解 抗體來源 Rabbit 克隆類型 Polyclonal 交叉反應 Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Rabbit, ) 產品應用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user. 分 子 量 78kDa 細胞定位 細胞核 細胞漿 細胞膜 性 狀 Liquid 濃 度 1mg/ml 免 疫 原 KLH conjugated synthesised phosphopeptide derived from rat 5-Lipoxygenase around the phosphorylation site of Ser663:YL(p-S)PD 亞 型 IgG 純化方法 affinity purified by Protein A 儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. PubMed PubMed 產品介紹 This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.
Function:
Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
Subunit:
Interacts with ALOX5AP and LTC4S.
Subcellular Location:
Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein.
Post-translational modifications:
Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.
Similarity:
Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain.
SWISS:
P09917
Gene ID:
25290
Database links:
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
5-脂氧合酶蛋白(5-LOX; 5-lipoxygenase; Arachidonate 5-lipoxygenase; 5-LO; ALOX5 )是花生四烯酸代謝途徑中的一種關鍵酶,在惡性腫瘤的發(fā)生、發(fā)展過程中起了重要作用.抑制5-脂氧合酶及其產物的表達有可能預防和逆轉惡性腫瘤的發(fā)生。 產品圖片 
Sample:
Heart (Rat) Lysate at 40 ug
Primary: Anti- Phospho-5-Lipoxygenase (Ser663) (bs-3252R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 75 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-5-Lipoxygenase (Ser663)) Polyclonal Antibody, Unconjugated (bs-3252R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-5-Lipoxygenase (Ser663)) Polyclonal Antibody, Unconjugated (bs-3252R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-5-Lipoxygenase(Ser663) Polyclonal Antibody, Unconjugated(bs-3252R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
