| 中文名稱 | 細胞分化周期CDC42蛋白抗體 |
| 別 名 | CDC 42; CDC42Hs; Cell division control protein 42 homolog; Cell division cycle 42; Cell division cycle 42 isoform 1; Cell division cycle 42 isoform 2; dJ224A6.1.1; dJ224A6.1.2; G25K; G25K GTP binding protein; Growth regulating protein; GTP binding protein 25kD; Small GTP binding protein CDC42; CDC42_HUMAN. |
| 研究領域 | 腫瘤 免疫學 細胞周期蛋白 轉錄調節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, ) |
| 產品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 25kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CDC42:101-191/191 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]. Function: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration. Subunit: The GTP-bound form interacts with CCPG1. Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, CDC42SE1, CDC42SE2, PARD6A, PARD6B and PARD6G (in a GTP-dependent manner). Interacts with activated CSPG4 and with BAIAP2. Interacts with Zizimin1/DOCK9 and Zizimin2/DOCK11, which activate it by exchanging GDP for GTP. Interacts with NET1 and ARHGAP33/TCGAP. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ. Interacts with USP6. May interact with ARHGEF16; responsible for the activation of CDC42 by the viral protein HPV16 E6. Interacts with NEK6. Part of a collagen stimulated complex involved in cell migration composed of CDC42, CRK, TNK2 and BCAR1/p130cas. Subcellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody. Post-translational modifications: AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo. Similarity: Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily. SWISS: P60953 Gene ID: 998 Database links: Entrez Gene: 998 Human Entrez Gene: 12540 Mouse Entrez Gene: 64465 Rat Omim: 116952 Human SwissProt: P60953 Human SwissProt: P60766 Mouse SwissProt: Q8CFN2 Rat Unigene: 467637 Human Unigene: 1022 Mouse Unigene: 447553 Mouse Unigene: 60067 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Cdc42，是細胞骨架和細胞極化的重要調節(jié)蛋白，在減數(shù)分裂和卵母細胞成熟過程中有重要的作用，尤其在細胞分化，遷移過程中起到一定的作用,目前在腫瘤研究中得到了重視。 |
| 產品圖片 |  Sample: Lymph node (Mouse) Lysate at 40 ugPrimary: Anti-CDC42 (bs-3555R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 28 kD  Sample:placenta (Mouse) Lysate at 40 ug Primary: Anti-CDC42 (bs-3555R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kD Observed band size: 25 kD  Tissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CDC42 Polyclonal Antibody, Unconjugated(bs-3555R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDC42) Polyclonal Antibody, Unconjugated (bs-3555R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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