| 中文名稱 | 磷酸丙糖異構(gòu)酶抗體 |
| 別 名 | TIM; TPI 1; TPI; TPI1; Triose phosphate isomerase 1; Triose phosphate isomerase; Triosephosphate isomerase 1; Triosephosphate isomerase; MGC88108; TPIS_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 27kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 細(xì)胞外基質(zhì) |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Triosephosphate isomerase:151-249/249 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq] Subunit: Homodimer. Post-translational modifications: The initiator methionine for isoform 2 is removed. DISEASE: Triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]: Autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the triosephosphate isomerase family. SWISS: P60174 Gene ID: 7167 Database links: UniProtKB/Swiss-Prot: P60174.3 Entrez Gene: 281543 Cow Entrez Gene: 7167 Human Entrez Gene: 21991 Mouse Entrez Gene: 24849 Rat Omim: 190450 Human SwissProt: Q5E956 Cow SwissProt: P54714 Dog SwissProt: P60174 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 磷酸丙糖異構(gòu)酶（Triose-phosphate isomerase，通常簡稱為TPI或TIM）是一種能夠催化丙糖磷酸異構(gòu)體在二羥丙酮磷酸和D型甘油醛-3-磷酸之間轉(zhuǎn)換。 磷酸丙糖異構(gòu)酶在糖酵解中具有重要作用，對于有效的能量生成是必不可少的。磷酸丙糖異構(gòu)酶被發(fā)現(xiàn)存在于幾乎所有的生物體，包括哺乳動物、昆蟲、真菌、植物和大多數(shù)細(xì)菌中。 如果人體缺乏磷酸丙糖異構(gòu)酶，會導(dǎo)致嚴(yán)重的神經(jīng)系統(tǒng)紊亂，該病癥被稱為磷酸丙糖異構(gòu)酶缺乏癥。 |
| 產(chǎn)品圖片 |  Sample: Muscle (Mouse) Lysate at 40 ugPrimary: Anti-Triosephosphate isomerasee (bs-4042R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 30 kD  Sample:293T(Human) Cell Lysate at 30 ug Primary: Anti- Triosephosphate isomerase (bs-4042R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kD  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TPIS Polyclonal Antibody, Unconjugated(bs-4042R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Positive control(blue): RSC96 cellsIsotype control(orange): Cell lines treated with rabbit IgG (bs-0295P)instead of the primary antibody to confirm that primary antibody binding is specific. primary antibody(Green):Anti-Triosephosphate isomerase antibody(bs-4042R),Concebtration: 5μg/10^6 cells Secondary only control(light blue): Both cell lines treated with Goat Anti-rabbit IgG/FITC antibody (bs-0295G-FITC) to confirm no background signal produced from secondary antibody alone. |
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