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長壽相關(guān)基因lASS6抗體

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中文名稱 長壽相關(guān)基因lASS6抗體
別    名 T1L; CerS 6; CerS6; LAG1 homolog ceramide synthase 6; LAG1 longevity assurance homolog 6; LASS 6; Longevity assurance homolog 6; MGC129949; MGC129950; CERS6_HUMAN.  
研究領(lǐng)域 腫瘤  細胞生物  免疫學(xué)  信號轉(zhuǎn)導(dǎo)  轉(zhuǎn)錄調(diào)節(jié)因子  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, )
產(chǎn)品應(yīng)用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 45kDa
細胞定位 細胞核 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human LASS6:31-130/384 
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產(chǎn)品介紹 The LASS (longevity-assurance homologue) family members, which are highly conserved among eukaryotes, function in ceramide synthesis. LASS6 (LAG1 homolog, ceramide synthase 6) may be involved in sphingolipid synthesis or its regulation.
Function:
May be involved in sphingolipid synthesis or its regulation
Subcellular Location:
Nucleus membrane; Multi-pass membrane protein (Potential). Endoplasmic reticulum membrane; Multi-pass membrane protein
Post-translational modifications:
Glycosylation on Asn-18 is not necessary for function
Similarity:
Contains 1 homeobox DNA-binding domain.
Contains 1 TLC (TRAM/LAG1/CLN8) domain.
SWISS:
Q6ZMG9
Gene ID:
253782
Database links:

Entrez Gene: 253782 Human

Entrez Gene: 241447 Mouse

Entrez Gene: 366065 Rat

SwissProt: Q6ZMG9 Human

SwissProt: Q8C172 Mouse

Unigene: 506829 Human

Unigene: 724377 Human

Unigene: 222222 Mouse

Unigene: 393211 Mouse

Unigene: 8190 Rat


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品圖片 Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LASS6) Polyclonal Antibody, Unconjugated (bs-5080R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LASS6) Polyclonal Antibody, Unconjugated (bs-5080R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human pancreatic carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LASS6) Polyclonal Antibody, Unconjugated (bs-5080R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-LASS6 Polyclonal Antibody, Unconjugated(bs-5080R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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