| 中文名稱 | 多聚免疫球蛋白受體抗體 |
| 別 名 | Hepatocellular carcinoma associated protein TB6; Phosphatidylinositol glycan, class R; PIgR; Poly Ig receptor; Polymeric immunoglobulin receptor; PIGR_HUMAN. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, (predicted: Mouse, Rat, Dog, Cow, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 81kDa |
| 細(xì)胞定位 | 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PIGR:31-130/764 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | This gene is a member of the immunoglobulin superfamily. The encoded poly-Ig receptor binds polymeric immunoglobulin molecules at the basolateral surface of epithelial cells; the complex is then transported across the cell to be secreted at the apical surface. A significant association was found between immunoglobulin A nephropathy and several SNPs in this gene.[provided by RefSeq, Sep 2009] Function: This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Secretory component: Secreted. Post-translational modifications: N-glycosylation is not necessary for Ig binding. Similarity: Contains 5 Ig-like V-type (immunoglobulin-like) SWISS: P01833 Gene ID: 5284 Database links: Entrez Gene: 5284 Human Entrez Gene: 18703 Mouse Omim: 173880 Human SwissProt: P01833 Human SwissProt: O70570 Mouse Unigene: 497589 Human Unigene: 276414 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 多聚免疫球蛋白受體（pIgR）屬Ⅰ型跨膜糖蛋白，可與多聚免疫球蛋白A和多聚免疫球蛋白M特異性結(jié)合，通過(guò)穿胞轉(zhuǎn)運(yùn)，將它們從上皮細(xì)胞基底側(cè)膜轉(zhuǎn)運(yùn)到頂膜，并最終分泌到外分泌液中去. 在此過(guò)程中，多聚免疫球蛋白受體的細(xì)胞外段被水解，釋放出與多聚免疫球蛋白A或多聚免疫球蛋白M相結(jié)合的細(xì)胞外段（又稱為分泌成分）. 分泌成分是sIgA分子的重要組成部分，直接參與sIgA的粘膜防御功能，而且在被動(dòng)粘膜免疫中也有重要作用. 多聚免疫球蛋白受體通過(guò)介導(dǎo)細(xì)胞內(nèi)多聚免疫球蛋白的轉(zhuǎn)運(yùn)，可以在粘膜的腔面阻止病原體粘附，在上皮細(xì)胞內(nèi)中和病毒，也可以將固有層內(nèi)的抗原分泌出去. |
| 產(chǎn)品圖片 |  Sample:HepG2(Human) Cell Lysate at 30 ug Primary: Anti-PIGR (bs-6061R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kD Observed band size: 131 kD  Sample:293T(Human) Cell Lysate at 30 ug Primary: Anti-PIGR (bs-6061R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kD Observed band size: 131 kD  Paraformaldehyde-fixed, paraffin embedded (Human hepatocellular carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PIGR) Polyclonal Antibody, Unconjugated (bs-6061R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minu Blank control : 293T Cells (Blue line)Isotype control: bs-0295P-AF647 (Orange line) Primary antibody: bs-6061R-AF647 (Green line) COncentration: 2μg/10^6 cells,4℃ incubate 30min. |
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