| 中文名稱 | 前細(xì)胞脹亡受體誘導(dǎo)膜損傷蛋白抗體 |
| 別 名 | KCT3; Keratinocytes associated transmembrane protein 3; Pro oncosis receptor inducing membrane injury; Serine/threonine rich receptor; TMEM123; Transmembrane protein 123; PORIM_HUMAN. |
| 研究領(lǐng)域 | 細(xì)胞生物 細(xì)胞凋亡 激酶和磷酸酶 細(xì)胞膜受體 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 19kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Porimin:101-180/208 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | This gene encodes a highly glycosylated transmembrane protein with a high content of threonine and serine residues in its extracellular domain, similar to a broadly defined category of proteins termed mucins. Exposure of some cell types to anti PORIMIN (pro oncosis receptor inducing membrane injury) antibody, crosslinks this protein on the cell surface and induces a type of cell death termed oncosis. Oncosis is distinct from apoptosis and is characterized by a loss of cell membrane integrity without DNA fragmentation. This gene product is proposed to function as a cell surface receptor that mediates cell death. Function: Implicated in oncotic cell death, characterized by cell swelling, organelle swelling, vacuolization and increased membrane permeability. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Ubiquitous. Not expressed in ovary. Expressed in keratinocytes. Similarity: Belongs to the CD164 family. SWISS: Q8N131 Gene ID: 114908 Database links: Entrez Gene: 114908 Human Omim: 606356 Human SwissProt: Q8N131 Human SwissProt: Q96QV2 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |  Sample:Skin (Mouse) Lysate at 40 ug Skin (Rat) Lysate at 40 ug Primary: Anti- Porimin (bs-7674R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19 kD Observed band size: 64 kD  Sample:Lymph node (Mouse) Lysate at 40 ug Primary: Anti- Porimin (bs-7674R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19 kD Observed band size: 74 kD  Sample:Embryo (Mouse) Lysate at 40 ug Primary: Anti- Porimin (bs-7674R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19 kD Observed band size: 74 kD  Blank control:Molt4.Primary Antibody (green line): Rabbit Anti-Porimin antibody (bs-7674R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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