| 中文名稱(chēng) | 9號(hào)染色體開(kāi)放閱讀框86抗體 |
| 別 名 | Chromosome 9 open reading frame 86; FLJ10101; Hypothetical protein LOC55684; KIAA1984; Parf; Partner of ARF; pp8875; Putative GTP binding protein Parf; Rab like GTP binding protein 1; Rab like GTP binding protein 1C; Rab like protein 1; RBEL1; RP11-216L13.9. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 表觀遺傳學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 80kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human C9orf86:631-729/729 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | C9orf86 (Partner of ARF;PARF) binds p19(ARF), but not the p16(INK4A) isoform of CDKN2A. Knockdown of C9orf86 by RNA interference reduced the growth inhibitory activity of p19(ARF) and enhanced the rate of cell proliferation. Function: May enhance cellular proliferation. May reduce growth inhibitory activity of CDKN2A. Subcellular Location: Cytoplasmic and Nuclear. Note=Predominantly cytoplasmic. Proposed to shuttle between cytoplasm and nucleus. Post-translational modifications: Isoform 1 is O-glycosylated, while other isoforms are not. Similarity: Belongs to the small GTPase superfamily. Rab family. SWISS: Q3YEC7 Gene ID: 55684 Database links: Entrez Gene: 55684 Human Entrez Gene: 227624 Mouse Entrez Gene: 227624 Rat Omim: 610615 Human SwissProt: Q3YEC7 Human SwissProt: Q5U3K5 Mouse Unigene: 370555 Human Unigene: 29600 Mouse Unigene: 443064 Mouse Unigene: 17709 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |  Sample:Stomach (Mouse) Lysate at 40 ug Primary: Anti- C9orf86 (bs-9545R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kD Observed band size: 80 kD  Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C9orf86) Polyclonal Antibody, Unconjugated (bs-9545R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C9orf86) Polyclonal Antibody, Unconjugated (bs-9545R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C9orf86) Polyclonal Antibody, Unconjugated (bs-9545R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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