| 中文名稱(chēng) | 小鼠血清白蛋白抗體 |
| 別 名 | Alb 1; ALB; ALBU_MOUSE; Albumin 1; Albumin; DKFZp779N1935; PRO0883; PRO0903; PRO1341; PRO2044; PRO2619; Serum albumin. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 發(fā)育生物學(xué) 干細(xì)胞 新陳代謝 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 68kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Mouse serum albumin: |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq, Jul 2008]. Function: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Subcellular Location: Secreted. Tissue Specificity: Plasma. Post-translational modifications: Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606. Glycated in diabetic patients. Phosphorylation sites are present in the extracellular medium. Acetylated on Lys-223 by acetylsalicylic acid. DISEASE: Familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]: Form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the ALB/AFP/VDB family. Contains 3 albumin domains. SWISS: P07724 Gene ID: 11657 Database links: Entrez Gene: 100379232 Guinea pig Entrez Gene: 100034206 Horse Entrez Gene: 213 Human Entrez Gene: 11657 Mouse Entrez Gene: 396960 Pig Entrez Gene: 100009195 Rabbit Entrez Gene: 24186 Rat Omim: 103600 Human SwissProt: P49064 Cat SwissProt: P19121 Chicken SwissProt: P02769 Cow SwissProt: P49822 Dog SwissProt: P35747 Horse SwissProt: P02768 Human SwissProt: P07724 Mouse SwissProt: P08835 Pig SwissProt: P49065 Rabbit SwissProt: P02770 Rat Unigene: 418167 Human Unigene: 16773 Mouse Unigene: 202968 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |  Sample:A549(Human) Cell Lysate at 30 ug Primary: Anti-Mouse serum albumin (bs-2256R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD  Sample:Lung (Mouse) Lysate at 40 ug Spleen (Mouse) Lysate at 40 ug Primary: Anti-Mouse serum albumin (bs-2256R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse Liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mouse serum albumin ) Polyclonal Antibody, Unconjugated (bs-2256R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mouse serum albumin) Polyclonal Antibody, Unconjugated (bs-2256R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining. |
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