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腺苷A2b受體/神經(jīng)生長(zhǎng)因子1受體抗體ADORA2B

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中文名稱 腺苷A2b受體/神經(jīng)生長(zhǎng)因子1受體抗體
別    名 Adenosine A2b Receptor; adora 2; adora 2b; ADORA2; ADORA2B; HGNC:264; Netrin 1 receptor; AA2BR_HUMAN.  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, Rat,  (predicted: Mouse, Dog, Rabbit, )
產(chǎn)品應(yīng)用 WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 37kDa
細(xì)胞定位 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ADORA2B:231-332/332 
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產(chǎn)品介紹 This gene encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008].

Function:
Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P29274

Gene ID:
136

Database links:

Entrez Gene: 136 Human

Omim: 600446 Human

SwissProt: P29275 Human

Unigene: 167046 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
產(chǎn)品圖片 Protein: mouse lung lysate;
Primary: rabbit Anti-ADORA2B (bs-10217R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:37 kD
Observed band size: 35 kDTissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADORA2B Polyclonal Antibody, Unconjugated(bs-10217R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat brain; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADORA2B Polyclonal Antibody, Unconjugated(bs-10217R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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