中文名稱 | 鈣依賴膜結合蛋白Copine蛋白1抗體 |
別 名 | Copine 1; Copine I; Copine-1; Copine1; CopineI; COPN 1; COPN1; CPN 1; CPN1; CPNE 1; CPNE1; CPNE1_HUMAN; MGC1142. |
研究領域 | 腫瘤 細胞生物 信號轉導 轉運蛋白 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Human, Mouse, (predicted: Rat, Pig, Cow, Sheep, ) |
產品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 59kDa |
細胞定位 | 細胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human CPNE1:1-120/537 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
PubMed | PubMed |
產品介紹 | Copine I is a member of the copine family of evolutionarily conserved, soluble, calcium-dependent, membrane-binding proteins. Members of the copine family are involved in signal transduction and membrane trafficking. Copine I is ubiquitously expressed and contains two N-terminal C2 domains and one C-terminal VWFA (von Willebrand factor A) domain, which is also referred to as the A domain or the core domain. As is characteristic of the copine family, copine I functions in membrane trafficking and is capable of binding phospholipids in a calcium-dependent manner. Copine I is also able to bind liposomes. Via its VWFA domain, copine I directly interacts with the ubiquitin-conjugating enzyme, UBC12, and may play a role in the regulation of TNF alpha-signaling. Function: May function in membrane trafficking. Exhibits calcium-dependent phospholipid binding properties. Subunit: Interacts with CCDC22. Similarity: Belongs to the copine family. Contains 2 C2 domains. Contains 1 VWFA domain. SWISS: Q99829 Gene ID: 615677 Database links: Entrez Gene: 615677 Cow Entrez Gene: 477213 Dog Entrez Gene: 8904 Human Entrez Gene: 266692 Mouse Entrez Gene: 100233174 Pig Entrez Gene: 100328904 Rabbit Omim: 604205 Human SwissProt: Q99829 Human SwissProt: Q8C166 Mouse Unigene: 246413 Human Unigene: 700188 Human Unigene: 27660 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
產品圖片 | Protein: spleen(mouse) lysate at 40ug; Primary: rabbit Anti-CPNE1 (bs-14024R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 59 kD Observed band size: 59 kDProtein: spleen(mouse) lysate at 40ug; Primary: rabbit Anti-CPNE1 (bs-14024R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 59 kD Observed band size: 59 kDTissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated(bs-14024R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated(bs-14024R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated(bs-14024R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated(bs-14024R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated(bs-14024R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated(bs-14024R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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