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中文名稱	磷酸化真核翻譯起始因子4B抗體
別名	eIF4B (phospho S406); p-eIF4B (phospho S406); EIF 4B; EIF-4B; Eukaryotic initiation factor 4B; IF4B_HUMAN.
產(chǎn)品類型	磷酸化抗體
研究領(lǐng)域	轉(zhuǎn)運(yùn)蛋白表觀遺傳學(xué)
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應(yīng)	Human, Mouse, Rat, (predicted: Dog, Cow, Horse, Sheep, )
產(chǎn)品應(yīng)用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	69kDa
細(xì)胞定位	細(xì)胞漿
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated Synthesised phosphopeptide derived from human EIF4B around the phosphorylation site of Ser406:HP(p-S)WR
亞型	IgG
純化方法	affinity purified by Protein A
儲(chǔ) 存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
產(chǎn)品介紹	eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot). Function: Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. Post-translational modifications: Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex. Similarity: Contains 1 RRM (RNA recognition motif) domain. SWISS: P23588 Gene ID: 1975 Database links: Entrez Gene: 1975 Human Entrez Gene: 75705 Mouse Entrez Gene: 300253 Rat Omim: 603928 Human SwissProt: P23588 Human SwissProt: Q8BGD9 Mouse Unigene: 648394 Human Unigene: 702041 Human Unigene: 290022 Mouse Unigene: 391795 Mouse Unigene: 95954 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品圖片	Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kD Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kD Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kD Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kD Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kD Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69kD Observed band size: 69kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-EIF4B (Ser406)) Polyclonal Antibody, Unconjugated (bs-14551R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-EIF4B (Ser406)) Polyclonal Antibody, Unconjugated (bs-14551R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.