中文名稱 | FAM124A蛋白抗體 |
別 名 | F124A_HUMAN; FAM124A; family with sequence similarity 124, member A; family with sequence similarity 124A; hypothetical protein LOC220108; Protein FAM124A. |
研究領(lǐng)域 | 細胞生物 免疫學 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Human, Mouse, (predicted: Rat, Chicken, Pig, Cow, Horse, Sheep, ) |
產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 60kDa |
細胞定位 | 細胞核 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human FAM124A:301-400/546 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
PubMed | PubMed |
產(chǎn)品介紹 | |
產(chǎn)品圖片 | Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-FAM124A(bs-14712R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60kD Observed band size: 63kD Sample: Ovary (Mouse) Lysate at 40 ug Primary: Anti-FAM124A(bs-14712R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60kD Observed band size: 60kD Sample: 293T Cell (Human) Lysate at 40 ug Primary: Anti-FAM124A(bs-14712R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60kD Observed band size: 63kD Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (THOC1) Polyclonal Antibody, Unconjugated (bs-14712R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
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