英文名稱 | FITM1 |
中文名稱 | FITM1蛋白抗體 |
別 名 | Fat inducing protein 1; Fat inducing transcript 1; Fat storage inducing transmembrane protein 1; Fat storage-inducing transmembrane protein 1; Fat-inducing protein 1; FIT1; FITM 1; Fitm1; FITM1_HUMAN; MGC46490. |
研究領(lǐng)域 | 細胞生物 免疫學 信號轉(zhuǎn)導 細胞類型標志物 中文名稱 | 核苷轉(zhuǎn)運蛋白ENT2抗體 | 別 名 | DER12; Equilibrative NBMPR-insensitive nucleoside transporter; Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter; Equilibrative nucleoside transporter 2; HNP36; Hydrophobic nucleolar protein, 36 kDa; SLC29A2; solute carrier family 29 (nucleoside transporters), member 29; Solute carrier family 29 member 2; S29A2_HUMAN. | 研究領(lǐng)域 | 腫瘤 細胞生物 神經(jīng)生物學 | 抗體來源 | Rabbit | 克隆類型 | Polyclonal | 交叉反應 | Mouse, Rat, (predicted: Human, ) | 產(chǎn)品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. | 分 子 量 | 50kDa | 細胞定位 | 細胞核 細胞膜 | 性 狀 | Liquid | 濃 度 | 1mg/ml | 免 疫 原 | KLH conjugated synthetic peptide derived from human ENT2:221-320/456 | 亞 型 | IgG | 純化方法 | affinity purified by Protein A | 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. | 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. | PubMed | PubMed | 產(chǎn)品介紹 | The uptake of nucleosides by transporters, such as SLC29A2, is essential for nucleotide synthesis by salvage pathways in cells that lack de novo biosynthetic pathways. Nucleoside transport also plays a key role in the regulation of many physiologic processes through its effect on adenosine concentration at the cell surface (Griffiths et al., 1997 [PubMed 9396714]).[supplied by OMIM, Nov 2008] Function: ENT2 mediates equilibrative transport of purine, pyrimidine nucleosides and the purine base hypoxanthine. It is less sensitive than ENT1 to inhibition by nitrobenzylthioinosine (NBMPR), dipyridamole, dilazep and draflazine. Subcellular Location: Basolateral cell membrane; Multi-pass membrane protein. Nucleus membrane; Multi-pass membrane protein. Note=Localized at the basolateral cell membrane in polarized MDCK cells. Tissue Specificity: Expressed in skeletal muscle, liver, lung, placenta, brain, heart, kidney and ovarian tissues. Similarity: Belongs to the SLC29A/ENT transporter (TC 2.A.57) family. SWISS: Q14542 Gene ID: 3177 Database links: Entrez Gene: 3177 Human Entrez Gene: 13340 Mouse Entrez Gene: 65194 Rat Omim: 602110 Human SwissProt: Q14542 Human SwissProt: Q61672 Mouse SwissProt: Q9JIT8 Mouse SwissProt: O54699 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. | 產(chǎn)品圖片 | Sample: Cerebrum (Mouse) Lysate at 40 ug Muscle (Mouse) Lysate at 40 ug Liver (Mouse) Lysate at 40 ug Primary: Anti- ENT2 (bs-10550R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD Protein: brain(mouse) lysate at 40ug; Primary: rabbit Anti-ENT2 (bs-10550R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 50 kD Observed band size: 55 kDTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ENT2 Polyclonal Antibody, Unconjugated(bs-10550R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ENT2 Polyclonal Antibody, Unconjugated(bs-10550R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining | |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Human, Rat, (predicted: Mouse, Dog, Cow, Horse, Rabbit, Sheep, ) |
產(chǎn)品應用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 32kDa |
細胞定位 | 細胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human FITM1:1-100/292 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
PubMed | PubMed |
產(chǎn)品介紹 | FIT1 belongs to an evolutionarily conserved family of proteins involved in fat storage (Kadereit et al., 2008 [PubMed 18160536]).[supplied by OMIM, May 2008] Function: Plays an important role in lipid droplet accumulation. Subcellular Location: Endoplasmic reticulum membrane. Tissue Specificity: Primarily expressed in heart and skeletal muscle. Similarity: Belongs to the FIT family. SWISS: A5D6W6 Gene ID: 161247 Database links: Entrez Gene: 161247 Human Entrez Gene: 68680 Mouse Omim: 612028 Human SwissProt: A7YWN2 Cow SwissProt: A5D6W6 Human SwissProt: Q91V79 Mouse SwissProt: B2MVP8 Pig Unigene: 128060 Human Unigene: 36758 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
產(chǎn)品圖片 | Protein: 293T(human) lysate at 40ug; Primary: rabbit Anti-FITM1 (bs-16090R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 32 kD Observed band size: 28 kD Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37∑C for 30min; Antibody incubation with (Fat inducing transcript; FITM1) Polyclonal Antibody, Unconjugated (bs-16090R) at 1:400 overnight at 4∑C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37∑C for 30min; Antibody incubation with (FITM1) Polyclonal Antibody, Unconjugated (bs-16090R) at 1:400 overnight at 4∑C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Fat inducing transcript; FITM1) Polyclonal Antibody, Unconjugated (bs-16090R) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining. |