中文名稱(chēng) | 三羧酸載體樣蛋白3抗體 |
別 名 | BA108L7.2; OTTHUMP00000059201; RP11-108L7.3; SFX 3; SFX3; SFXN3_HUMAN; SFXN 3; sideroflexin 3; TCC; Tricarboxylate carrier; Tricarboxylate carrier like protein. |
研究領(lǐng)域 | 腫瘤 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 新陳代謝 |
抗體來(lái)源 | Rabbit |
克隆類(lèi)型 | Polyclonal |
交叉反應(yīng) | Human, Mouse, Rat, (predicted: Dog, Cow, Horse, Rabbit, Sheep, ) |
產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復(fù)) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 36kDa |
細(xì)胞定位 | 細(xì)胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human SFXN3:221-325/325 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
PubMed | PubMed |
產(chǎn)品介紹 | SFXN3, or sideroflexin 3, is a potential iron transporter. Function: Potential iron transporter. Subcellular Location: Mitochondrion membrane; Multi-pass membrane protein Similarity: Belongs to the sideroflexin family. SWISS: Q9BWM7 Gene ID: 81855 Database links: Entrez Gene: 81855 Human Entrez Gene: 511755 Cow Entrez Gene: 94280 Mouse Entrez Gene: 65042 Rat SwissProt: A6QP55 Cow SwissProt: Q9BWM7 Human SwissProt: Q91V61 Mouse SwissProt: Q9JHY2 Rat Unigene: 283844 Human Unigene: 36169 Mouse Unigene: 37545 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
產(chǎn)品圖片 | Sample: HepG2 Cell (Human) Lysate at 30 ug Primary: Anti-SFXN3 (bs-17308R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SFXN3) Polyclonal Antibody, Unconjugated (bs-17308R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SFXN3 Polyclonal Antibody, Unconjugated(bs-17308R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SFXN3 Polyclonal Antibody, Unconjugated(bs-17308R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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