| 中文名稱 | 環(huán)指蛋白193抗體 |
| 別 名 | bcl-2 ARE RNA binding protein; mex 3 homolog D (C. elegans); MEX 3D; mex3; OK/SW-cl.4; ring finger (C3HC4 type) and KH domain containing 1; Ring finger and KH domain containing 1; RKHD1; RNF193; tino. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 結(jié)合蛋白 表觀遺傳學(xué) 環(huán)指蛋白 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 65kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MEX3D/RNF193:201-300/651 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Mex3d is a ubiquitously expressed protein. Due to alternative splicing events truncating the N-terminus, a variant form of Mex3d exists and is known as TINO. Function: RNA binding protein, may be involved in post-transcriptional regulatory mechanisms. Subcellular Location: Cytoplasm Nucleus Tissue Specificity: Ubiquitously expressed in all the cell lines and tissues tested. Post-translational modifications: Phosphorylated Similarity: Contains 2 KH domains. Contains 1 RING-type zinc finger. SWISS: Q86XN8 Gene ID: 399664 Database links: Entrez Gene: 399664 Human Omim: 611009 Human SwissProt: Q86XN8 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |  Sample: U251 Cell (Human) Lysate at 40 ugPrimary: Anti-MEX3D/RNF193 (bs-18822R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 62 kD  Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF193) Polyclonal Antibody, Unconjugated (bs-18822R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF193) Polyclonal Antibody, Unconjugated (bs-18822R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF193) Polyclonal Antibody, Unconjugated (bs-18822R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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