| 中文名稱 | 神經(jīng)元素2抗體 |
| 別 名 | Atoh4; Math4A; MGC; MGC46562; NEUROG2; Neurogenin2; NGN2; Protein atonal homolog 4. |
| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 神經(jīng)生物學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse, Rat, (predicted: Human, Chicken, Dog, Cow, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 30kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Neurogenin 2:101-200/272 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | Neurogenin 2 is a helix-loop-helix class of transcription factor. Transcription factors with bHLH motifs modulate critical events in the development of the mammalian neocortex. The transition from proliferation to neurogenesis involves a coordinate increase in the activity of proneural bHLH factors, including Neurogenin 2 and a decrease in the activity of Hes and Id factors. bHLH factors have key roles in corticogenesis, affecting the timing of differentiation and the specification of cell fate. Function: Transcriptional regulator. Involved in neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Subunit: Efficient DNA binding requires dimerization with another bHLH protein. Subcellular Location: Nucleus. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. SWISS: Q9H2A3 Gene ID: 63973 Database links: Entrez Gene: 63973 Human Entrez Gene: 11924 Mouse Entrez Gene: 295475 Rat Omim: 606624 Human SwissProt: Q9H2A3 Human SwissProt: P70447 Mouse Unigene: 567563 Human Unigene: 42017 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |  Sample:Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Neurogenin 2 (bs-3692R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Neurogenin 2 Polyclonal Antibody, Unconjugated(bs-3692R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neurogenin 2) Polyclonal Antibody, Unconjugated (bs-3692R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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