| 中文名稱 | 嗜中性粒細(xì)胞胞漿因子1抗體 |
| 別 名 | 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NCF 47K; NCF-1; NCF-47K; Ncf1; NCF1_HUMAN; Neutrophil cytosol factor 1; Neutrophil cytosolic factor 1; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox organizing protein 2; Nox-organizing protein 2; NOXO2; p47 phox; p47-phox; SH3 and PX domain containing protein 1A; SH3 and PX domain-containing protein 1A; SH3PXD1A. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, (predicted: Rat, Dog, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1ug/test not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 45kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NCF1:151-250/390 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | NCF1, along with NCF2 and a membrane bound cytochrome b558, is required for activation of the latent NADPH oxidase necessary for superoxide production. Defects in NCF1 are the cause of autosomal cytochrome-b-positive chronic granulomatous disease type 1 (CGD). Function: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). Subunit: Interacts with NOXA1. Interacts with ADAM15. Interacts with TRAF4. Interacts with FASLG. Subcellular Location: Cytoplasm. Post-translational modifications: Phosphorylated by PRKCD; phosphorylation induces activation of NCF1 and NADPH oxidase activity. DISEASE: Granulomatous disease, chronic, cytochrome-b-positive 1, autosomal recessive (CGD1) [MIM:233700]: A disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Contains 1 PX (phox homology) domain. Contains 2 SH3 domains. SWISS: P14598 Gene ID: 653361 Database links: Entrez Gene: 281345 Cow Entrez Gene: 653361 Human Entrez Gene: 17969 Mouse Entrez Gene: 100134857 Pig Entrez Gene: 100008803 Rabbit Entrez Gene: 114553 Rat Omim: 608512 Human SwissProt: O77774 Cow SwissProt: P14598 Human SwissProt: Q09014 Mouse Unigene: 647047 Human Unigene: 655201 Human Unigene: 425296 Mouse Unigene: 38575 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |  Sample:Bone (Mouse) Lysate at 40 ug Lymph node (Mouse) Lysate at 40 ug Spleen (Mouse) Lysate at 40 ug Primary: Anti-NCF1 (bs-3886R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 48 kD  Sample:Lane 1: Lung (Mouse) Lysate at 40 ug Lane 2: Spleen (Mouse) Lysate at 40 ug Lane 3: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-NCF1 (bs-3886R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD  U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NCF1 Antibody(bs-3886R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange). |
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