| 中文名稱 | 蛋白磷酸酶2調(diào)節(jié)亞基5E抗體 |
| 別 名 | 2A5E_HUMAN; Epsilon isoform of regulatory subunit B56 protein phosphatase 2A; PP2A B subunit B' epsilon; PP2A B subunit B' epsilon isoform; PP2A B subunit B56 epsilon; PP2A B subunit B56 epsilon isoform; PP2A B subunit isoform B''-epsilon; PP2A B subunit isoform B'-epsilon; PP2A B subunit isoform B56-epsilon; PP2A B subunit isoform PR61-epsilon; PP2A B subunit isoform R5-epsilon; PP2A B subunit PR61 epsilon; PP2A B subunit PR61 epsilon isoform; PP2A B subunit R5 epsilon; PP2A B subunit R5 epsilon isoform; PPP2R5E; Protein phosphatase 2 regulatory subunit B (B56) epsilon isoform; Protein phosphatase 2 regulatory subunit B' epsilon isoform; Regulatory subunit B of protein phosphatase 2 epsilon isoform; Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit epsilon. |
| 研究領(lǐng)域 | 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse, Rat, (predicted: Human, Horse, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 55kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PPP2R5E:121-220/467 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | The protein encoded by this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an epsilon isoform of the regulatory subunit B56 subfamily. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2013] Function: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Subcellular Location: Cytoplasm. Post-translational modifications: Phosphorylated on serine residues. Similarity: Belongs to the phosphatase 2A regulatory subunit B56 family. SWISS: Q16537 Gene ID: 5529 Database links: Entrez Gene: 5529 Human Entrez Gene: 26932 Mouse Entrez Gene: 299147 Rat Omim: 601647 Human SwissProt: Q16537 Human SwissProt: Q61151 Mouse Unigene: 334868 Human Unigene: 259626 Mouse Unigene: 440646 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |  Sample:Thymus (Mouse) Lysate at 40 ug Primary: Anti- PPP2R5E (bs-19971R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD  Sample:Testis (Mouse) Lysate at 40 ug Primary: Anti- PPP2R5E (bs-19971R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD  Sample:Cerebrum (Mouse) Lysate at 40 ug Epithelium (Mouse) Lysate at 40 ug Primary: Anti-PPP2R5E(bs-19971R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55kD Observed band size: 55kD  Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP2R5E) Polyclonal Antibody, Unconjugated (bs-19971R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. |
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