| 中文名稱 | 水通道蛋白-3抗體 |
| 別 名 | AQP 3; AQP3; Aquaporin 3 (GIL blood group); Aquaporin 3 (Gill blood group); Aquaporin3; Aquaporin-3; GIL; Gill blood group; AQP3_MOUSE |
| 研究領(lǐng)域 | 腫瘤 信號(hào)轉(zhuǎn)導(dǎo) 通道蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 32kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse AQP3:201-292/292 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. [provided by RefSeq, Aug 2011] Function: Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Renal medulla and colon. Predominantly in the inner medulla. Expressed in basal layer of epidermal keratinocytes. Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family. SWISS: Q8R2N1 Gene ID: 11828 Database links: Entrez Gene: 360 Human Entrez Gene: 11828 Mouse Entrez Gene: 65133 Rat Omim: 600170 Human SwissProt: Q92482 Human SwissProt: Q8R2N1 Mouse SwissProt: P47862 Rat Unigene: 234642 Human Unigene: 34043 Mouse Unigene: 11109 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 通道蛋白（Channel Protein） AQP 3主要分布于腎臟集合管上皮細(xì)胞基側(cè)質(zhì)膜,除了對水有通透性外、還可轉(zhuǎn)運(yùn)尿素和甘油等小分子物質(zhì),而尿素在尿液濃縮機(jī)制中有著重要的作用. 有學(xué)者認(rèn)為：AQP-3的陽性率與某些腫瘤分化程度有關(guān)（胃癌、結(jié)腸癌、乳腺癌、前列腺癌、子宮癌、卵巢癌、甲狀腺癌等） |
| 產(chǎn)品圖片 |  Sample: Lung (mouse) Lysate at 40 ugPrimary: Anti- AQP3 (bs-1253R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32kD Observed band size: 27kD  Tissue/cell: rat taste buds; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AQP-3 Polyclonal Antibody, Unconjugated(bs-1253R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control: Hela.Primary Antibody (green line): Rabbit Anti-AQP3 antibody (bs-1253R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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