| 中文名稱 | 細胞表面趨化因子受體2抗體 |
| 別 名 | C-C Chemokine receptor 2B; C C chemokine receptor type 2; C C CKR 2; CC chemokine receptor type 2; CC CKR 2; CCCKR2; CCR 2; CCR1L; CCR2A; CCR2B; CCR5L; CD192; CD192 antigen; Chemokine C C motif receptor 2; Chemokine CC Motif Receptor 2; CKR 2; CKR2; CKR2A; CKR2B; CMKBR2; MCP 1 R; MCP1 RECEPTOR; MCP1R; Monocyte chemoattractant protein 1 receptor; Monocyte Chemotactic Protein 1 Receptor; CCR2_HUMAN; C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CCR-2; CCR2; Monocyte chemoattractant protein 1 receptor; MCP-1-R. |
| 研究領域 | 免疫學 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, (predicted: Mouse, Rat, Dog, Horse, ) |
| 產品應用 | ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=0.2μg/Test IF=1:100-500 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 41kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CCR-2:201-300/373 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產品介紹 | This gene encodes two isoforms of a receptor for monocyte chemoattractant protein-1, a chemokine which specifically mediates monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. The receptors encoded by this gene mediate agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. This gene is located in the chemokine receptor gene cluster region. Two alternatively spliced transcript variants are expressed by the gene. [provided by RefSeq, Mar 2009]. Function: Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. Subunit: Binds to HIV-1 Tat. Interacts with ARRB1. Subcellular Location: Cell membrane; Multi-pass membrane protein. Post-translational modifications: N-glycosylated. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P41597 Gene ID: 729230 Database links: Entrez Gene: 729230 Human Entrez Gene: 12772 Mouse Entrez Gene: 60463 Rat Omim: 601267 Human SwissProt: P41597 Human SwissProt: P51683 Mouse SwissProt: O55193 Rat Unigene: 511794 Human Unigene: 705362 Human Unigene: 6272 Mouse Unigene: 211983 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CCR-2是屬于G蛋白偶聯(lián)受體一家族，參與調節(jié)白細胞的遷移和活性作用，是細胞膜蛋白，它的變異與HIV-1抵抗有關。CCR-2不僅在宮頸癌組織中表達，而且在人其他腫瘤如白血病、淋巴瘤、乳腺癌、腎B 癌、胃癌、結腸癌、肝癌和子宮癌中均有過度表達，可作為肝癌及乳腺癌診斷的標志物。 |
| 產品圖片 |  Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CCR2 Polyclonal Antibody, Unconjugated(bs-0562R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control: Raw264.7.Primary Antibody (green line): Rabbit Anti-CCR-2 antibody (bs-0562R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.  Blank control (blue line): Hela (blue).Primary Antibody (green line): Rabbit Anti- CCR-2 antibody (bs-0562R) Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 80% methanol for 5 min at -20℃ (The cells were fixed with 2% paraformaldehyde for 10 min at room temperature). Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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