| 來源 | 用途 | 交叉反應性 | 分子量 |
| Rabbit | WB, ICC, IHC, IF | H, M, R | 27KDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當調整)：
| WB | IP | IF | IHC | ICC | FC | ELISA | ChIP |
| 1:500-1:1000 | - | 1:100 | 1:200 | 1:100 | - | - | - |
抗體詳細信息如下:
| About this Antibody | |
| Name | HSP27 Rabbit Polyclonal Antibody |
| Category | Polyclonal antibody(pAb); Primary antibody |
| Isotype | IgG |
| Purification | Peptide affinity purified |
| About the Immunogen | |
| Immunogen | This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to HSP27. |
| Gene ID | 3315 |
| SwissProt | P04792 |
| Synonyms | HSPB1;CMT2F;DKFZp586P1322;HMN2B;HS.76067;HSP27;HSP28;Hsp25;SRP27 |
| Category | Protein Folding and Trafficking |
| Background | Hsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, α-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. Hsp27 appears in many cell types, especially all types of muscle cells. It is located mainly in the cytosol, but also in the perinuclear region, endoplasmatic reticulum, and nucleus. It is overexpressed during different stages of cell differentiation and development. This suggests an essential role for Hsp27 in the differentiation of tissues. Probably Hsp27 – among other chaperones – is involved in the process of cell differentiation. Changes of Hsp27 levels were observed in Ehrlich ascite cells, embryonic stem cells, normal B-cells, B-lymphoma cells, osteoblasts, keratinocytes, neurons etc. |
包裝清單：
| 產(chǎn)品編號 | 產(chǎn)品名稱 | 包裝 |
| AF0183 | HSP27 Rabbit Polyclonal Antibody | 50μl |
| AZ050 | Western一抗稀釋液 | 50ml |
| — | 說明書 | 1份 |
保存條件：
HSP27 Rabbit Polyclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項：
如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體�；厥盏目贵w通常至少可以重復使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細胞化學(ICC)等適當用途。如果希望獲得最佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品僅限于專業(yè)人員的科學研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內。
為了您的安全和健康，請穿實驗服并戴一次性手套操作。
使用說明：
請根據(jù)抗體的實際用途選擇相應的使用方法。
1. Western檢測：
A. 按照推薦的稀釋比例用提供的Western一抗稀釋液稀釋抗體。
B. 把經(jīng)過封閉的蛋白膜與稀釋好的一抗4℃緩慢搖動過夜或室溫緩慢搖動2小時，確保稀釋的抗體至少能在搖動的瞬間覆蓋蛋白膜。
C. 回收稀釋的一抗，4℃保存以備下次繼續(xù)使用。
D. 按照Western的實驗步驟進行后續(xù)的洗滌、二抗孵育、洗滌和檢測等操作。
2. 免疫染色：
可以使用生產(chǎn)的免疫染色一抗稀釋液(P0103)稀釋抗體，使用后注意回收稀釋好的一抗，
3. 其它實驗操作請自行參考適當?shù)膒rotocol進行。
4. 代表性圖片：
 |  |
| Fig. 1. Western blot analysis of Hsp27 on different cell lysates using anti- Hsp27 antibody at 1/500 dilution. Positive control:Lane 1: Hela Lane 2: Jurkat Lane 3: 293 Lane 4: Human kidney Lane 5: Mouse kidney Lane 6: Mouse heart. | Fig. 2. Immunocytochemistry staining Hsp27 in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS. |
 |  |
| Fig. 3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Hsp27 antibody. Counter stained with hematoxylin. | Fig. 4. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Hsp27 antibody. Counter stained with hematoxylin. |
