| 來源 | 用途 | 交叉反應(yīng)性 | 分子量 |
| Rabbit | WB, IP, IF, IHC, ICC | H, M, R | 11/33 kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當(dāng)用途時(shí)的一抗稀釋。
建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
| WB | IP | IF | IHC | ICC | FC | ELISA | ChIP |
| 1:1,000-1:2,000 | 1:10-1:50 | 1:50-1:200 | 1:50-1:200 | 1:50-1:200 | - | - | - |
抗體詳細(xì)信息如下:
| About this Antibody | |
| Name | Caspase-6 Rabbit Monoclonal Antibody |
| Category | Rabbit Monoclonal Antibody (RabMAb); Primary antibody |
| Isotype | IgG |
| Purification | Affinity purification |
| About the Immunogen | |
| Immunogen | Recombinant protein |
| Gene ID | 839(Human); 12368(Mouse); 83584(Rat) |
| SwissProt | P55212(Human); O08738(Mouse); O35397(Rat) |
| Synonyms | MCH2 |
| Category | Apoptosis |
| Background | A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is comprised of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6, caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9 and caspase-10. Ced-3/ caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly(ADP-ribose)polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins, which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli. |
包裝清單:
| 產(chǎn)品編號(hào) | 產(chǎn)品名稱 | 包裝 |
| AF1927 | Caspase-6 Rabbit Monoclonal Antibody | 50μl |
| AZ050 | Western一抗稀釋液 | 50ml |
| - | 說明書 | 1份 |
保存條件:
Caspase-6 Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會(huì)導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng):
如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn)，請(qǐng)注意回收使用過的稀釋抗體�；厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請(qǐng)4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得最佳的檢測效果，請(qǐng)考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品僅限于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
代表性圖片：
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| Fig. 1. Western blot analysis of Caspase-6 on different lysates using anti-Caspase-6 antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse colon; Lane 2: MCF-7 | Fig. 2. ICC staining Caspase-6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS. |
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| Fig. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Caspase-6 antibody. Counter stained with hematoxylin. |
