英文名稱Cytokeratin 8
中文名稱細(xì)胞角蛋白8單克隆抗體
別 名card2; Cardiac autoantigen 2 120kD; CK 8; CK8; CK-8; ck8; Cyk 8; cyk8; CYKER; Cytokeratin endo A; Cytokeratin-8; Cytokeratin8; DreK8; EndoA; k0; CYK8; k2c8; K2C8_HUMAN; k8; Keratin 8; Keratin type ii cytoskeletal 8; Keratin, type II cytoskeletal 8; Keratin-8; Keratin8; KO; Krt 2.8; Krt 8; krt8; KRT-8; MGC118110; MGC174782; MGC53564; MGC85764; sb:cb186; Type-II keratin Kb8.
研究領(lǐng)域腫瘤 細(xì)胞生物 信號轉(zhuǎn)導(dǎo)
抗體來源Mouse
克隆類型Monoclonal
克 隆 號10A8
交叉反應(yīng)Human, Mouse, Rat,
產(chǎn)品應(yīng)用IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:200-800 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量53kDa
細(xì)胞定位細(xì)胞核 細(xì)胞漿 細(xì)胞膜
性 狀Liquid
濃 度1mg/ml
免 疫 原Recombinant human CK8:
亞 型IgG
純化方法affinity purified by Protein G
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012].
Function:
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Subunit:
Heterotetramer of two type I and two type II keratins. KRT8 associates with KRT18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP. Interacts with APEX1.
Subcellular Location:
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
Tissue Specificity:
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Post-translational modifications:
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
DISEASE:
Defects in KRT8 are a cause of cirrhosis (CIRRH) [MIM:215600].
Similarity:
Belongs to the intermediate filament family.
SWISS:
P05787
Gene ID:
3856
Database links:
Entrez Gene: 3856 Human
Entrez Gene: 16691 Mouse
Entrez Gene: 25626 Rat
Omim: 148060 Human
SwissProt: P05787 Human
SwissProt: P11679 Mouse
SwissProt: Q10758 Rat
Unigene: 533782 Human
Unigene: 708445 Human
Unigene: 358618 Mouse
Unigene: 11083 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
結(jié)構(gòu)蛋白（Structural Proteins）
上皮細(xì)胞胞質(zhì)中的骨架蛋白除微絲（肌動蛋白）、微管蛋白外，主要是細(xì)胞角蛋白（cytokeratins，CK）。CK8也是上皮細(xì)胞的特征性標(biāo)記物：CK8存在于某些正常腺上皮及其腫瘤，包括許多導(dǎo)管上皮和腺上皮，如結(jié)腸、胃、小腸、氣管的上皮和尿路上皮。CK8主要用于腺癌和導(dǎo)管癌的診斷，鱗狀細(xì)胞癌一般不表達(dá)CK8。
| 產(chǎn)品圖片 |  Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-33061M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.  Paraformaldehyde-fixed, paraffin embedded (mouse liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-33061M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-33061M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-33061M) at 1:500 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (bs-0296G-CY3) for 90 minutes, and DAPI for nuclei staining.  Paraformaldehyde-fixed, paraffin embedded (Human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-33061M) at 1:500 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (bs-0296G-FITC) for 90 minutes, and DAPI for nuclei staining.  Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-33061M) at 1:500 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (bs-0296G-CY3) for 90 minutes, and DAPI for nuclei staining.  Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-33061M) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (bs-0296G-FITC) for 90 minutes, and DAPI for nuclei staining. |
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