英文名稱S100B
中文名稱S100B蛋白單克隆抗體
別 名NEF; Protein S100 B; Protein S100-B; S 100 calcium binding protein beta chain; S 100 protein beta chain; S 100 protein beta subunit; S-100 protein beta chain; S-100 protein subunit beta; S100; S100 calcium binding protein B; S100 calcium binding protein beta (neural); S100 calcium binding protein beta chain; S100 calcium-binding protein B; S100 protein beta chain; S100B_HUMAN; S100beta; S100 β; S100-β; S100β.
研究領(lǐng)域腫瘤 細(xì)胞生物 神經(jīng)生物學(xué) 細(xì)胞周期蛋白 細(xì)胞分化
抗體來源Mouse
克隆類型Monoclonal
克 隆 號(hào)1C6
交叉反應(yīng)Human, Mouse, Rat,
產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量10kDa
細(xì)胞定位細(xì)胞核 細(xì)胞漿
性 狀Liquid
濃 度1mg/ml
免 疫 原Recombinant human S100B protein: 2-92/92aa (N-6x His-Tag)
亞 型IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹S100 beta is a member of the S100 family of proteins containing 2 EF-hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
Function:
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.
Subunit:
Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38 (By similarity). The S100B dimer interacts with two molecules of CAPZA1.
Subcellular Location:
Cytoplasm. Nucleus.
Tissue Specificity:
Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
Similarity:
Belongs to the S-101 family.
Contains 2 EF-hand domains.
SWISS:
P04271
Gene ID:
6285
Database links:
Entrez Gene: 6285 Human
Omim: 176990 Human
SwissProt: P04271 Human
Unigene: 422181 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
結(jié)構(gòu)蛋白（Structural Proteins）
S100B是一組可溶性、相對低分子質(zhì)量、高酸性蛋白，屬神經(jīng)膠質(zhì)細(xì)胞特異性蛋白，亦可存在于神經(jīng)纖維中。在研究腸神經(jīng)系統(tǒng)的形態(tài)特征及結(jié)構(gòu)特征中，S100B有良好的表達(dá)功能。 S100B蛋白其生理作用尚不清楚，可能與細(xì)胞內(nèi)鈣離子媒介的調(diào)節(jié)作用有關(guān)。S100B蛋白可存在于神經(jīng)鞘瘤，成軟骨細(xì)胞瘤、軟骨肉瘤、骨肉瘤、神經(jīng)膠質(zhì)瘤、郎格罕斯細(xì)胞瘤、一些癌腫和黑素細(xì)胞腫瘤等。
在免疫組化中，除了惡性黑素瘤皮損，S100B蛋白還在良性黑素細(xì)胞痣中增加，如：藍(lán)痣、普通后天痣、雀斑樣痣、Spitz痣、氣球狀細(xì)胞痣、色素性梭形細(xì)胞痣和發(fā)育不良痣中表達(dá)。還有報(bào)道S100B蛋白的染色強(qiáng)度與腫瘤細(xì)胞的黑素含量呈負(fù)相關(guān)。結(jié)締組織增生性的或親神經(jīng)的黑素瘤不易染色。
| 產(chǎn)品圖片 |  Sample: Lane 1: Cerebral cortex (Rat) Lysate at 40 ug Lane 2: Spinal cord (Mouse) Lysate at 40 ug Lane 3: Spinal cord (Rat) Lysate at 40 ug Lane 4: Cerebellum (Mouse) Lysate at 40 ug Lane 5: Cerebellum (Rat) Lysate at 40 ug Primary: Anti-S100B (bsm-10832M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 11 kD Observed band size: 9 kD  Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (ascites of bsm-10832M 3C2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.  Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (ascites of bsm-10832M 3C2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.  Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (ascites of bsm-10832M 3C2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.  Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (ascites of bsm-10832M 3C2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.  Paraformaldehyde-fixed, paraffin embedded (Human neurofibroma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M 1C6) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M 1C6) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining. |
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