英文名稱GAP43
中文名稱神經(jīng)生長相關(guān)蛋白43單克隆抗體
別 名Growth Associated Protein-43; Neuromodulin; Axonal membrane protein GAP 43; B-50; F1; GAP 43; Growth Associated Protein 43; Nerve Growth Related Peptide; Neural phosphoprotein B 50; Neuromodulin; GAP-43; pp46; NEUM_HUMAN; Protein F1; QtrA-11580; QtrA-13071.
研究領(lǐng)域腫瘤 免疫學(xué) 神經(jīng)生物學(xué)
抗體來源Mouse
克隆類型Monoclonal
克 隆 號6F11
交叉反應(yīng)Mouse, Rat, (predicted: Human, )
產(chǎn)品應(yīng)用WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量43kDa
細(xì)胞定位細(xì)胞漿 細(xì)胞膜 分泌型蛋白
性 狀Liquid
濃 度1mg/ml
免 疫 原Recombinant human GAP43 Protein:
亞 型IgG
純化方法affinity purified by Protein G
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function:
This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.
Subunit:
Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence.
Subcellular Location:
Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane; Peripheral membrane protein. Note=Cytoplasmic surface of growth cone and synaptic plasma membranes.
Post-translational modifications:
Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2.
Similarity:
Belongs to the neuromodulin family.
Contains 1 IQ domain.
SWISS:
P17677
Gene ID:
2596
Database links:
Entrez Gene: 2596 Human
Entrez Gene: 14432 Mouse
Entrez Gene: 29423 Rat
GenBank: NP_002036 Human
Omim: 162060 Human
SwissProt: P17677 Human
SwissProt: P06837 Mouse
SwissProt: P07936 Rat
Unigene: 134974 Human
Unigene: 1222 Mouse
Unigene: 10928 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
神經(jīng)生物學(xué)相關(guān)蛋白（Neurobiology）；神經(jīng)標(biāo)志物
GAP43 （Growth associated protein-43）又稱作neuromodulin，是一個軸突膜蛋白，是一種神經(jīng)特異性的蛋白質(zhì)，參與神經(jīng)細(xì)胞外生長及突觸發(fā)育形成和神經(jīng)細(xì)胞再生。在神經(jīng)元發(fā)育和再生過程中以高水平表達(dá)。能調(diào)解軸突延伸作用，改變細(xì)胞形態(tài)。作為細(xì)胞內(nèi)信號，可大大增強(qiáng)與G蛋白偶聯(lián)的受體轉(zhuǎn)運(yùn)作用。
神經(jīng)生長相關(guān)蛋白-GAP-43和神經(jīng)細(xì)胞黏附因子(neural cell adhesion molecule,NCAM)與突觸可塑性密切相關(guān).GAP-43是一種神經(jīng)細(xì)胞膜上的特異性磷蛋白,在神經(jīng)發(fā)育和再生過程中呈現(xiàn)高表達(dá),被作為突觸生長的標(biāo)志物,有稱脊髓生長相關(guān)蛋白
GAP-43與CaM結(jié)合,參與G蛋白相互作用,神經(jīng)遞質(zhì)的釋放,作用于胞吞/胞吐過程,通過小囊溶合或誘導(dǎo)生長錐和突觸前末端的胞吞促進(jìn)膜擴(kuò)展,與海馬長時程增強(qiáng)密切相關(guān).
神經(jīng)細(xì)胞黏附因子是細(xì)胞表面糖蛋白大家族的成員之一,促進(jìn)軸突生長,對長時記憶的保持有重要影響,同時,GAP-43對其具調(diào)節(jié)作用，廣泛而深入地研究GAP-43意義深遠(yuǎn)。
| 產(chǎn)品圖片 |  Sample: BV2 (Mouse) Lysate at 40 ug VSC4.1(Rat) Lysate at 40 ug Primary: Anti-GAP43 (bsm-33192M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 43 kD  Sample: Spinal cord(Mouse) Lysate at 40 ug Spinal cord(Rat) Lysate at 40 ug Primary: Anti-GAP43 (bsm-33192M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 43-46 kD Observed band size: 43 kD  Sample: Cerebrum(Rat) Lysate at 40 ug Cerebellum(Rat) Lysate at 40 ug Primary: Anti-GAP43 (bsm-33192M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 43-46 kD Observed band size: 43 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAP43) Monoclonal Antibody, Unconjugated (bs-33192M-8A8) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.  Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAP43) Monoclonal Antibody, Unconjugated (bs-33192M-8A8) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.  Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAP43) Monoclonal Antibody, Unconjugated (bsm-33192M ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. |
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