產(chǎn)品類型內(nèi)參抗體 

研究領域細胞生物  免疫學  線粒體  

抗體來源Mouse

克隆類型Monoclonal

克 隆 號8D8

交叉反應Human, Mouse, Rat, 

產(chǎn)品應用WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:200-800 （石蠟切片需做抗原修復）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量17kDa

細胞定位細胞漿 細胞膜 線粒體

性    狀Liquid

濃    度1mg/ml

免 疫 原Recombinant human COX4: 

亞    型IgG

純化方法affinity purified by Protein G

儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

產(chǎn)品介紹Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq, Jul 2008]

Function:

This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

Subcellular Location:

Mitochondrion inner membrane.

Tissue Specificity:

Ubiquitous.

Similarity:

Belongs to the cytochrome c oxidase IV family.

SWISS:

P13073

Gene ID:

1327

Database links:

Entrez Gene: 1327 Human

Entrez Gene: 12857 Mouse

Entrez Gene: 29445 Rat

Entrez Gene: 281090 Cow

Omim: 123864 Human

SwissProt: P00423 Cow

SwissProt: Q3SZS0 Cow

SwissProt: P13073 Human

SwissProt: P19783 Mouse

SwissProt: P10888 Rat

Unigene: 433419 Human

Unigene: 386758 Mouse

Unigene: 2528 Rat

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

細胞色素c氧化酶4亞型1抗體；（細胞色素c氧化酶IV亞型1）是一種存在線粒體上的大型跨膜蛋白復合物。由于細胞色素氧化酶是呼吸電子傳遞鏈的第四個中心酶復合物，因此又被稱為復合物IV。

產(chǎn)品圖片

Sample: Jurkat Cell (Human) Lysate at 40 ug Primary: Anti- COX4 (bsm-33037M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 16 kD Sample: HepG2 Cell (Human) Lysate at 40 ug Primary: Anti- COX4 (bsm-33037M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 16 kD Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COX4) Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COX4) Monoclonal Antibody, Unconjugated (bsm-33037M-8D8) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COX4) Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (bs-0296G-CY3) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COX4) Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (bs-0296G-FITC) for 90 minutes, and DAPI for nuclei staining.