| Synonyms: | EFM-192C（人乳腺癌細(xì)胞）EFM-192C |
| Background: | established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old Caucasian woman with breast adenocarcinoma; sister cell lines EFM-192A and -192B show differences in molecular aspects; erbB-2 gene is amplified and overexpressed |
| Species: | human (Homo sapiens) |
| Tissue: | breast |
| Disease: | breast carcinoma |
| Gender: | N/A |
| Morphology: | epitheloid adherent cells growing as monolayers |
| Growth Mode: | N/A |
| Doubling Time: | ca. 4 days |
| DNA Profile: | N/A Cell Line Authentication Service |
| Culture Medium: | RPMI-1640+20%FBS We strongly suggest to purchase the complete medium from |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin - |
| Fingerprint: | same DNA profile as EFM-192A/EFM-192B using multiplex PCR of minisatellite markers |
| Species: | confirmed as human with IEF of AST, MDH, NP |
| Cytogenetics: | highly rearranged human hypohexaploid karyotype with 10% polyploidy - 122-138<6n>XXXXX/XXXXXX - related to EFM-192A and EFM-192 B |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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