| Synonyms: | GOS-3GOS-3(人膠質(zhì)細(xì)胞細(xì)胞) |
| Background: | originally described as a glioma cell line established from the tumor material of brain from a 55-year-old man with a mixed astro-oligodendroglioma (grade II/III); however, DNA fingerprinting at DSMZ showed clearly that this cell line is in reality a derivative of the human glioblastoma cell line U-343-MG; described in the literature to express neuroepithelial markers GFAP, vimentin and S-100 protein as well as pp60C-SRC, but not the neuro-specific variant pp60C-SRCN and to express neuropoietic factors CNTF, LIF and their receptors; LIF and oncostatin M induce growth inhibition and differentiation |
| Species: | Homo sapiens, human |
| Tissue: | brain |
| Disease: | mixed astro-oligodendroglioma |
| Gender: | famale,55 years |
| Morphology: | glioblastoma |
| Growth Mode: | fibroblastoid, adherent cells growing as monolayer |
| Doubling Time: | 50 hours |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 12 D16S539: 9,11 D5S818: 12,13 D7S820: 8,12 THO1: 7 TPOX: 9,11 vWA: 17 Cell Line Authentication Service |
| Culture Medium: | DMEM+10%FBS+4 mM L-glutamine We strongly suggest to purchase the complete medium |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays Cytogenetics: human hypodiploid karyotype with 20% polypoidy - 44(40-45)<2n>XXY/XXYY, -6, +7, -10, -14, -22, der(1)del(1)(p21)t(1;9)(q42;q34), der(9)t(9;16)(p13;p11)t(1;9)(q42;q34), der(16)t(9;16)(p13;p11), der(18)t(6;18)(p12;q12.2) - gain of 7 and loss of 10 and 22 typical in low-grade astrocytoma-oligodendroglioma - gains of sex chromosomes (particularly Y) are very rare in tumor cells Immunology: cytokeratin -, desmin -, endothel -, GFAP +, neurofilament -, vimentin + Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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