WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當調(diào)整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ELISA |
| 1:500-1:2000 | - | - | - | - | - | - | 1:1000-1:5000 |
抗體詳細信息如下:
| About this Antibody | |
| Name | SARS-CoV-2 N Protein Polyclonal Antibody |
| Category | Rabbit Polyclonal Antibody (pAb); Primary antibody |
| Isotype | IgG |
| Purification method | Antigen affinity purified |
| Positive samples | - |
| Cellular location | - |
| Customer validation | - |
| About the Immunogen | |
| Immunogen | Recombinant full length of SARS-CoV-2 Nucleocapsid Protein |
| Sequence | - |
| Gene ID | 43740575 |
| Swiss Prot | - |
| Synonyms | N protein; Nucleocapsid phosphoprotein; Nucleocapsid protein; N structural protein |
| Category | Anti-infection |
| Background | SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. SARS-CoV-2 Nucleocapsid Protein is associated with nucleic acid. It is the most abundant protein for coronavirus. Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the strong immunogenicity of coronavirus Nucleocapsid, it is believed that SARS-CoV-2 Nucleocapsid Protein has potential value for the diagnosis of the virus. |
包裝清單：
| 產(chǎn)品編號 | 產(chǎn)品名稱 | 包裝 |
| AF0325 | SARS-CoV-2 N Protein Polyclonal Antibody | 50µl |
| AZ050 | Western一抗稀釋液 | 50ml |
| — | 說明書 | 1份 |
保存條件：
SARS-CoV-2 N Protein Polyclonal antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項：
如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體�；厥盏目贵w通常至少可以重復使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細胞化學(ICC)等適當用途。如果希望獲得最佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品僅限于專業(yè)人員的科學研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的安全和健康，請穿實驗服并戴一次性手套操作
請根據(jù)抗體的實際用途選擇相應的使用方法。
1.Western檢測：
a.按照推薦的稀釋比例用提供的Western一抗稀釋液稀釋抗體。
b.把經(jīng)過封閉的蛋白膜與稀釋好的一抗4℃緩慢搖動過夜或室溫緩慢搖動2小時，確保稀釋的抗體至少能在搖動的瞬間覆蓋蛋白膜。
c.回收稀釋的一抗，4℃保存以備下次繼續(xù)使用。
d.按照Western的實驗步驟進行后續(xù)的洗滌、二抗孵育、洗滌和檢測等操作。具體操作可以
2.免疫染色：
可以使用生產(chǎn)的稀釋抗體，使用后注意回收稀釋好的一抗，
3.其它實驗操作請自行參考適當?shù)膒rotocol進行。
4.代表性圖片：
Fig. 1. Western blot using SARS-CoV-2 N Protein Polyclonal Antibody shows detection of a 48 kDa band corresponding to the protein.
Fig. 2. ELISA analysis of with SARS-CoV-2 N Protein Polyclonal Antibody. Antigen (1μg/mL). The antigen was used as the coating antigen, and the SARS-CoV-2 N Protein Polyclonal Antibody was used as the capture antigen for ELISA.
