| Species | Gene ID | Accession | EC | Source | Length | MW | Tag |
| Human | 59272 | Q9BYF1 | 3.4.17.23 | HEK293 | 598aa (w/o tag) | ~120kDa | hFc |
| 蛋白信息(About this protein) | |
| 名稱(chēng)(Name) | Recombinant Human ACE2 Protein (hFc Tag)；Recombinant Human ACE2 Protein (hFc Tag) (重組人血管緊張素轉(zhuǎn)換酶2蛋白，hFc Tag) |
| 別名(Synonyms) | Angiotensin I converting enzyme 2, ACE-Related Carboxypeptidase, Angiotensin-Converting Enzyme Homolog, ACEH, Metalloprotease MPROT15 |
| 產(chǎn)品簡(jiǎn)介(Background) | A DNA sequence encoding the mature extracellular domain (Gln18-Asp 615) of human ACE2 precursor (NP_068576.1) was expressed with the fused Fc region of human IgG4 (Glu98-Gly325) at the C-terminus. ACE2 belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. ACE2 is known to be expressed in various human organs, and its organ- and cell-specific expression suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2, the latter is the causative agent of coronavirus disease-2019 (COVID-19). Multiple splice variants have been found for this gene and the dACE2 (or MIRb-ACE2) splice variant has been found to be interferon inducible. |
| 產(chǎn)品用途(Applications) | 用于血管緊張素轉(zhuǎn)換酶2抑制劑的篩選、酶活性研究、抗體或結(jié)構(gòu)研究 |
| 外觀(Physical appearance) | 液體 |
| 活性(Biological activity) | ≥7,800U/mg (按定義1) 或7,800,000U/mg (按定義2) |
| 活力單位(Unit definition) | 定義1：溫度37℃條件下，每分鐘催化生成1nmol MCA的酶量定義為一個(gè)單位(Unit, U)。 定義2：溫度37℃條件下，在一分鐘內(nèi)裂解1pmol熒光肽底物MCA-APK(Dnp)-OH所需的酶量定義為一個(gè)單位(Unit, U)。 |
| 濃度(Concentration) | 1mg/ml |
| 純度(Purity) | ≥95% by SDS-PAGE |
| 配方(Formulation) | 0.1M phosphate buffer, 40% glycerol, pH8.0 |
| 使用方法(Recommended usage) | 用于抑制劑篩選時(shí)，一般每個(gè)反應(yīng)體系(200μl)約需要1ng-1μg本蛋白。 |
| 氨基酸序列(Amino acid sequence) | QSTIEEQAKT FLDKFNHEAE DLFYQSSLAS WNYNTNITEE NVQNMNNAGD KWSAFLKEQS TLAQMYPLQE IQNLTVKLQL QALQQNGSSV LSEDKSKRLN TILNTMSTIY STGKVCNPDN PQECLLLEPG LNEIMANSLD YNERLWAWES WRSEVGKQLR PLYEEYVVLK NEMARANHYE DYGDYWRGDY EVNGVDGYDY SRGQLIEDVE HTFEEIKPLY EHLHAYVRAK LMNAYPSYIS PIGCLPAHLL GDMWGRFWTN LYSLTVPFGQ KPNIDVTDAM VDQAWDAQRI FKEAEKFFVS VGLPNMTQGF WENSMLTDPG NVQKAVCHPT AWDLGKGDFR ILMCTKVTMD DFLTAHHEMG HIQYDMAYAA QPFLLRNGAN EGFHEAVGEI MSLSAATPKH LKSIGLLSPD FQEDNETEIN FLLKQALTIV GTLPFTYMLE KWRWMVFKGE IPKDQWMKKW WEMKREIVGV VEPVPHDETY CDPASLFHVS NDYSFIRYYT RTLYQFQFQE ALCQAAKHEG PLHKCDISNS TEAGQKLFNM LRLGKSEPWT LALENVVGAK NMNVRPLLNY FEPLFTWLKD QNKNSFVGWS TDWSPYAD 備注：本氨基酸序列僅供參考，不含F(xiàn)c序列，實(shí)際氨基酸長(zhǎng)度或序列可能有一定變化。 |
包裝清單：
| 產(chǎn)品編號(hào) | 產(chǎn)品名稱(chēng) | 包裝 |
| P2325-50μg | Recombinant Human ACE2 Protein (hFc Tag) | 50μg |
| P2325-200μg | Recombinant Human ACE2 Protein (hFc Tag) | 200μg |
| P2325-1mg | Recombinant Human ACE2 Protein (hFc Tag) | 1mg |
| － | 說(shuō)明書(shū) | 1份 |
保存條件：
-20℃保存，一年有效。
注意事項(xiàng)：
收到產(chǎn)品后請(qǐng)立即按照說(shuō)明書(shū)推薦的條件保存。在打開(kāi)管蓋前，請(qǐng)適當(dāng)離心，使附著在管蓋或管壁上的蛋白聚集于管底。
本產(chǎn)品僅限于專(zhuān)業(yè)人員的科學(xué)研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
使用說(shuō)明：
2.本產(chǎn)品經(jīng)SDS-PAGE電泳檢測(cè)的效果參考圖1。
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